Avidin-biotin complexation for enzyme sensor applications

Jun ichi Anzai, Tomonori Hoshi, Tetsuo Osa

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


The use of an avidin-biotin system in the preparation of enzyme sensors is described. The biotin-labelled enzymes can be immobilized on the avidin-modified electrode surface through avidin-biotin complexation. The various techniques for the surface derivatization with biotin and avidin and for the coupling with enzymes are cited. The possibility of constructing a protein architecture on the electrode is also discussed: this is based on the non-covalent interaction of avidin and biotin.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalTrAC - Trends in Analytical Chemistry
Issue number5
Publication statusPublished - 1994 May


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