Avidin-biotin complexation for enzyme sensor applications

Jun ichi Anzai; Tomonori Hoshi; Tetsuo Osa

doi:10.1016/0165-9936(94)85040-2

Avidin-biotin complexation for enzyme sensor applications

Jun ichi Anzai, Tomonori Hoshi, Tetsuo Osa

HOSP - Clinical Research, Innovation and Education Center

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Abstract

The use of an avidin-biotin system in the preparation of enzyme sensors is described. The biotin-labelled enzymes can be immobilized on the avidin-modified electrode surface through avidin-biotin complexation. The various techniques for the surface derivatization with biotin and avidin and for the coupling with enzymes are cited. The possibility of constructing a protein architecture on the electrode is also discussed: this is based on the non-covalent interaction of avidin and biotin.

Original language	English
Pages (from-to)	205-210
Number of pages	6
Journal	TrAC - Trends in Analytical Chemistry
Volume	13
Issue number	5
DOIs	https://doi.org/10.1016/0165-9936(94)85040-2
Publication status	Published - 1994 May

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AU - Hoshi, Tomonori

AU - Osa, Tetsuo

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AB - The use of an avidin-biotin system in the preparation of enzyme sensors is described. The biotin-labelled enzymes can be immobilized on the avidin-modified electrode surface through avidin-biotin complexation. The various techniques for the surface derivatization with biotin and avidin and for the coupling with enzymes are cited. The possibility of constructing a protein architecture on the electrode is also discussed: this is based on the non-covalent interaction of avidin and biotin.

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Avidin-biotin complexation for enzyme sensor applications

Abstract

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