@article{b7f245dbb9744005a15becdd9dc0e8b8,
title = "Basolateral sorting of the Mg2+ transporter CNNM4 requires interaction with AP-1A and AP-1B",
abstract = "Ancient conserved domain protein/cyclin M (CNNM) 4 is an evolutionarily conserved Mg2+ transporter that localizes at the basolateral membrane of the intestinal epithelia. Here, we show the complementary importance of clathrin adaptor protein (AP) complexes AP-1A and AP-1B in basolateral sorting of CNNM4. We first confirmed the basolateral localization of both endogenous and ectopically expressed CNNM4 in Madin-Darby Canine Kidney cells, which form highly polarized epithelia in culture. Single knockdown of μ1B, a cargo-recognition subunit of AP-1B, did not affect basolateral localization, but simultaneous knockdown of the μ1A subunit of AP-1A abrogated localization. Mutational analyses showed the importance of three conserved dileucine motifs in CNNM4 for both basolateral sorting and interaction with μ1A and μ1B. These results imply that CNNM4 is sorted to the basolateral membrane by the complementary function of AP-1A and AP-1B.",
keywords = "AP-1A, AP-1B, Basolateral sorting, CNNM4, Dileucine motif, Mg transport",
author = "Yusuke Hirata and Yosuke Funato and Hiroaki Miki",
note = "Funding Information: We thank Dr. H. F{\"o}lsch (Northwestern University) for providing the cDNAs for mouse μ1A and human μ1B, and the anti-μ1B rabbit polyclonal antibody, and Drs. M. Itoh (Dokkyo Medical University) and M. Furuse (National Institute for Physiological Sciences) for providing the anti-ZO-1 antibody. We are also grateful to T. Kasashima for technical assistance. Y.H. was supported by the Taniguchi Memorial Fellowship project funded by the Research Foundation for Microbial Diseases of Osaka University . This study was supported by Funding Program for Next Generation World-Leading Researchers from Japan Society for the Promotion of Science (JSPS) to H.M. (LS083), Exciting Leading-Edge Research Projects from Osaka University to H.M., and Grants-in-Aid for Scientific Research from JSPS and Ministry of Education, Culture, Sports, Science and Technology-Japan to H.M. ( 26111007 and 26291042 ) and Y.F. ( 22770195 and 23117710 ). The funding sources had no role in study design, data collection, data analysis, data interpretation, writing of the report, or decision to submit the report for publication. Publisher Copyright: {\textcopyright} 2014 Elsevier Inc.",
year = "2014",
month = dec,
day = "12",
doi = "10.1016/j.bbrc.2014.10.138",
language = "English",
volume = "455",
pages = "184--189",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3-4",
}