Bat coronaviruses and experimental infection of bats, the Philippines

Shumpei Watanabe; Joseph S. Masangkay; Noriyo Nagata; Shigeru Morikawa; Tetsuya Mizutani; Shuetsu Fukushi; Phillip Alviola; Tsutomu Omatsu; Naoya Ueda; Koichiro Iha; Satoshi Taniguchi; Hikaru Fujii; Shumpei Tsuda; Maiko Endoh; Kentaro Kato; Yukinobu Tohya; Shigeru Kyuwa; Yasuhiro Yoshikawa; Hiroomi Akashi

doi:10.3201/eid1608.100208

Bat coronaviruses and experimental infection of bats, the Philippines

Shumpei Watanabe, Joseph S. Masangkay, Noriyo Nagata, Shigeru Morikawa, Tetsuya Mizutani, Shuetsu Fukushi, Phillip Alviola, Tsutomu Omatsu, Naoya Ueda, Koichiro Iha, Satoshi Taniguchi, Hikaru Fujii, Shumpei Tsuda, Maiko Endoh, Kentaro Kato, Yukinobu Tohya, Shigeru Kyuwa, Yasuhiro Yoshikawa, Hiroomi Akashi

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

141 Citations (Scopus)

Abstract

Fifty-two bats captured during July 2008 in the Philippines were tested by reverse transcription-PCR to detect bat coronavirus (CoV) RNA. The overall prevalence of virus RNA was 55.8%. We found 2 groups of sequences that belonged to group 1 (genus Alphacoronavirus) and group 2 (genus Betacoronavirus) CoVs. Phylogenetic analysis of the RNA-dependent RNA polymerase gene showed that groups 1 and 2 CoVs were similar to Bat-CoV/China/A515/2005 (95% nt sequence identity) and Bat-CoV/HKU9-1/China/2007 (83% identity), respectively. To propagate group 2 CoVs obtained from a lesser dog-faced fruit bat (Cynopterus brachyotis), we administered intestine samples orally to Leschenault rousette bats (Rousettus leschenaulti) maintained in our laboratory. After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection.

Original language	English
Pages (from-to)	1217-1223
Number of pages	7
Journal	Emerging Infectious Diseases
Volume	16
Issue number	8
DOIs	https://doi.org/10.3201/eid1608.100208
Publication status	Published - 2010

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Watanabe, S., Masangkay, J. S., Nagata, N., Morikawa, S., Mizutani, T., Fukushi, S., Alviola, P., Omatsu, T., Ueda, N., Iha, K., Taniguchi, S., Fujii, H., Tsuda, S., Endoh, M., Kato, K., Tohya, Y., Kyuwa, S., Yoshikawa, Y., & Akashi, H. (2010). Bat coronaviruses and experimental infection of bats, the Philippines. Emerging Infectious Diseases, 16(8), 1217-1223. https://doi.org/10.3201/eid1608.100208

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title = "Bat coronaviruses and experimental infection of bats, the Philippines",

abstract = "Fifty-two bats captured during July 2008 in the Philippines were tested by reverse transcription-PCR to detect bat coronavirus (CoV) RNA. The overall prevalence of virus RNA was 55.8%. We found 2 groups of sequences that belonged to group 1 (genus Alphacoronavirus) and group 2 (genus Betacoronavirus) CoVs. Phylogenetic analysis of the RNA-dependent RNA polymerase gene showed that groups 1 and 2 CoVs were similar to Bat-CoV/China/A515/2005 (95% nt sequence identity) and Bat-CoV/HKU9-1/China/2007 (83% identity), respectively. To propagate group 2 CoVs obtained from a lesser dog-faced fruit bat (Cynopterus brachyotis), we administered intestine samples orally to Leschenault rousette bats (Rousettus leschenaulti) maintained in our laboratory. After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection.",

author = "Shumpei Watanabe and Masangkay, {Joseph S.} and Noriyo Nagata and Shigeru Morikawa and Tetsuya Mizutani and Shuetsu Fukushi and Phillip Alviola and Tsutomu Omatsu and Naoya Ueda and Koichiro Iha and Satoshi Taniguchi and Hikaru Fujii and Shumpei Tsuda and Maiko Endoh and Kentaro Kato and Yukinobu Tohya and Shigeru Kyuwa and Yasuhiro Yoshikawa and Hiroomi Akashi",

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doi = "10.3201/eid1608.100208",

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Watanabe, S, Masangkay, JS, Nagata, N, Morikawa, S, Mizutani, T, Fukushi, S, Alviola, P, Omatsu, T, Ueda, N, Iha, K, Taniguchi, S, Fujii, H, Tsuda, S, Endoh, M, Kato, K, Tohya, Y, Kyuwa, S, Yoshikawa, Y & Akashi, H 2010, 'Bat coronaviruses and experimental infection of bats, the Philippines', Emerging Infectious Diseases, vol. 16, no. 8, pp. 1217-1223. https://doi.org/10.3201/eid1608.100208

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T1 - Bat coronaviruses and experimental infection of bats, the Philippines

AU - Watanabe, Shumpei

AU - Masangkay, Joseph S.

AU - Nagata, Noriyo

AU - Morikawa, Shigeru

AU - Mizutani, Tetsuya

AU - Fukushi, Shuetsu

AU - Alviola, Phillip

AU - Omatsu, Tsutomu

AU - Ueda, Naoya

AU - Iha, Koichiro

AU - Taniguchi, Satoshi

AU - Fujii, Hikaru

AU - Tsuda, Shumpei

AU - Endoh, Maiko

AU - Kato, Kentaro

AU - Tohya, Yukinobu

AU - Kyuwa, Shigeru

AU - Yoshikawa, Yasuhiro

AU - Akashi, Hiroomi

PY - 2010

Y1 - 2010

N2 - Fifty-two bats captured during July 2008 in the Philippines were tested by reverse transcription-PCR to detect bat coronavirus (CoV) RNA. The overall prevalence of virus RNA was 55.8%. We found 2 groups of sequences that belonged to group 1 (genus Alphacoronavirus) and group 2 (genus Betacoronavirus) CoVs. Phylogenetic analysis of the RNA-dependent RNA polymerase gene showed that groups 1 and 2 CoVs were similar to Bat-CoV/China/A515/2005 (95% nt sequence identity) and Bat-CoV/HKU9-1/China/2007 (83% identity), respectively. To propagate group 2 CoVs obtained from a lesser dog-faced fruit bat (Cynopterus brachyotis), we administered intestine samples orally to Leschenault rousette bats (Rousettus leschenaulti) maintained in our laboratory. After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection.

AB - Fifty-two bats captured during July 2008 in the Philippines were tested by reverse transcription-PCR to detect bat coronavirus (CoV) RNA. The overall prevalence of virus RNA was 55.8%. We found 2 groups of sequences that belonged to group 1 (genus Alphacoronavirus) and group 2 (genus Betacoronavirus) CoVs. Phylogenetic analysis of the RNA-dependent RNA polymerase gene showed that groups 1 and 2 CoVs were similar to Bat-CoV/China/A515/2005 (95% nt sequence identity) and Bat-CoV/HKU9-1/China/2007 (83% identity), respectively. To propagate group 2 CoVs obtained from a lesser dog-faced fruit bat (Cynopterus brachyotis), we administered intestine samples orally to Leschenault rousette bats (Rousettus leschenaulti) maintained in our laboratory. After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated. Fruit bats infected with virus did not show clinical signs of infection.

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Bat coronaviruses and experimental infection of bats, the Philippines

Abstract

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