Bioassay using living cells integrated on a chip

Yu Suke Torisawa, Hitoshi Shiku, Tomoyuki Yasukawa, Tomokazu Matsue

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)


A living cell-based assay is widely used for the characterization of various cellular functions, screening of drugs and environmental monitoring. Recent progress in cell culture and micro-fabrication technologies has attracted the integration of cell culture and sensors on a chip. A bioassay using a cell chip makes it possible to evaluate small-volume samples with multitudinous characterizations, and is applicable for a rapid and simple detection system. This paper reviews cell-based bioassay using living cells integrated on a chip. The cell patterning and three-dimensional cell culture facilitates the integration of cell culture technology. These in vitro culture technologies are appropriate for the recapitulation of in vivo-like cell behavior, because of the potential to control the cell differentiation and polarity. Alternatively, the evaluation methods, such as cellular acidification, oxygen consumption and impedance measurements, are principally non-invasive, and are especially expected in the field of anticancer drug sensitivity test using a biopsy tumor.

Original languageEnglish
Pages (from-to)369-382
Number of pages14
JournalBunseki Kagaku
Issue number5
Publication statusPublished - 2004 May


  • 3-dimensional cell culture
  • Anticancer drug sensitivity test
  • Cell chip
  • Cell patterning
  • Cell-based assay
  • LAPS
  • Microphysiometer


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