The structural ordering of ZnO nanoparticles into a flower-type morphology was discussed. A peptide with both an affinity for ZnO and the ability to generate ZnO nanoparticles that assemble into a highly ordered flower-type structure was identified using a combinatorial library approach. The selected peptide recognizes ZnO, but not ZnS or Eu2O3. ZnO can be selectively immobilized on a substrate in neutral solution at room temperature by the ZnO-recognizing peptide. The peptide can condense Zn(OH)2 sol, and the conjugate of the ZnO-recognizing peptide with glycine linker (GGGSC) enables the homogeneous creation of a flower-type morphology composed of ZnO nanoparticles.