Biofuel cell anode: NAD+/glucose dehydrogenase-coimmobilized ketjenblack electrode

T. Miyake, M. Oike, S. Yoshino, Y. Yatagawa, K. Haneda, H. Kaji, M. Nishizawa

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


We have studied the coimmobilization of glucose dehydrogenase (GDH) and its cofactor, oxidized nicotinamide adenine dinucleotide (NAD+), on a ketjenblack (KB) electrode as a step toward a biofuel cell anode that works without mediators. A KB electrode was first treated with a sulfuric acid/nitric acid/water mixture to lower the overvoltage for NADH oxidation, and was next chemically modified with NAD+ and GDH. The improved GDH/NAD+/KB electrode is found to oxidize glucose around 0 V vs. Ag/AgCl. A biofuel cell constructed with a bilirubin oxidase-immobilized KB cathode showed a maximum power density of 52 μW/cm2 at 0.3 V.

Original languageEnglish
Pages (from-to)123-126
Number of pages4
JournalChemical Physics Letters
Issue number1-3
Publication statusPublished - 2009 Sept 28


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