Biogenic action of Lactobacillus plantarum SBT2227 promotes sleep in Drosophila melanogaster

Taro Ko, Hiroki Murakami, Azusa Kamikouchi, Hiroshi Ishimoto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Lactic acid bacteria (LAB) influence multiple aspects of host brain function via the production of active metabolites in the gut, which is known as the pre/probiotic action. However, little is known about the biogenic effects of LAB on host brain function. Here, we reported that the Lactobacillus plantarum SBT2227 promoted sleep in Drosophila melanogaster. Administration of SBT2227 primarily increased the amount of sleep and decreased sleep latency at the beginning of night-time. The sleep-promoting effects of SBT2227 were independent of the existing gut flora. Furthermore, heat treatment or mechanical crushing of SBT2227 did not suppress the sleep-promoting effects, indicative of biogenic action. Transcriptome analysis and RNAi mini-screening for gut-derived peptide hormones revealed the requirement of neuropeptide F, a homolog of the mammalian neuropeptide Y, for the action of SBT2227. These biogenic effects of SBT2227 on the host sleep provide new insights into the interaction between the brain and gut bacteria.

Original languageEnglish
Article number104626
Issue number7
Publication statusPublished - 2022 Jul 15
Externally publishedYes


  • Microbiology
  • Microbiome
  • Omics

ASJC Scopus subject areas

  • General


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