@article{793b0fdb5ebd447f85ff35601154d710,
title = "Biogenic action of Lactobacillus plantarum SBT2227 promotes sleep in Drosophila melanogaster",
abstract = "Lactic acid bacteria (LAB) influence multiple aspects of host brain function via the production of active metabolites in the gut, which is known as the pre/probiotic action. However, little is known about the biogenic effects of LAB on host brain function. Here, we reported that the Lactobacillus plantarum SBT2227 promoted sleep in Drosophila melanogaster. Administration of SBT2227 primarily increased the amount of sleep and decreased sleep latency at the beginning of night-time. The sleep-promoting effects of SBT2227 were independent of the existing gut flora. Furthermore, heat treatment or mechanical crushing of SBT2227 did not suppress the sleep-promoting effects, indicative of biogenic action. Transcriptome analysis and RNAi mini-screening for gut-derived peptide hormones revealed the requirement of neuropeptide F, a homolog of the mammalian neuropeptide Y, for the action of SBT2227. These biogenic effects of SBT2227 on the host sleep provide new insights into the interaction between the brain and gut bacteria.",
keywords = "Microbiology, Microbiome, Omics",
author = "Taro Ko and Hiroki Murakami and Azusa Kamikouchi and Hiroshi Ishimoto",
note = "Funding Information: We thank Dr. T. Awasaki, Dr. S. D. Pletcher, Dr. T. Sakai, Bloomington Drosophila Stock Center, and Vienna Drosophila Resource Center, Kyoto stock center for fly stocks. We also thank Dr. I. Mori for comments and discussion, Y.Arai and Y. Ishikawa for fly maintenance. This work was supported by Grant-in-Aid for Scientific research (C) (15K07147 and 18K06332 to HI), Inamori Foundation Research Grant, Japan to HI. and Research donation from Megmilk Snow Brand Co. Ltd. T.K. H.M. and H.I. designed the research; T.K. H.M. and H.I. performed the research; H.I. contributed new reagents/analytic tools; T.K. H.M. and H.I. analyzed the data; T.K. and H.I. wrote the article; and A.K. and H.I. reviewed and edit the article. All authors read and approved the article. T.K. and H.M. are employees of Megmilk Snow Brand Co. Ltd. The other authors declare no competing interest. Funding Information: We thank Dr. T. Awasaki, Dr. S. D. Pletcher, Dr. T. Sakai, Bloomington Drosophila Stock Center, and Vienna Drosophila Resource Center, Kyoto stock center for fly stocks. We also thank Dr. I. Mori for comments and discussion, Y.Arai and Y. Ishikawa for fly maintenance. This work was supported by Grant-in-Aid for Scientific research (C) ( 15K07147 and 18K06332 to HI), Inamori Foundation Research Grant, Japan to HI., and Research donation from Megmilk Snow Brand Co., Ltd . Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jul,
day = "15",
doi = "10.1016/j.isci.2022.104626",
language = "English",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "7",
}