Bioimaging using bipolar electrochemical microscopy with improved spatial resolution

Tomoki Iwama; Kumi Y. Inoue; Hiroya Abe; Tomokazu Matsue; Hitoshi Shiku

doi:10.1039/d0an00912a

Bioimaging using bipolar electrochemical microscopy with improved spatial resolution

Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku

Tohoku University

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

In this study, we developed bipolar electrochemical microscopy (BEM) using a closed bipolar electrode (cBPE) array with an electrochemiluminescence (ECL) detecting system. Because cBPEs are not directly connected to a detector, high spatio-temporal resolution imaging can be achieved by fabricating a microelectrode array in which each electrode point is arranged in a short interval. A cBPE array with individual cBPEs arranged in 41 μm intervals was successfully fabricated by depositing gold in the pores of a track-etched membrane using electroless plating. Using BEM with the cBPE array, which has a higher density of electrode points than the conventional multi-electrode array, we effectively demonstrated the imaging of [Fe(CN)6]3- diffusion and the respiratory activity of MCF-7 spheroids with high spatio-temporal resolution.

Original language	English
Pages (from-to)	6895-6900
Number of pages	6
Journal	The Analyst
Volume	145
Issue number	21
DOIs	https://doi.org/10.1039/d0an00912a
Publication status	Published - 2020 Nov 7

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10.1039/d0an00912a

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title = "Bioimaging using bipolar electrochemical microscopy with improved spatial resolution",

abstract = "In this study, we developed bipolar electrochemical microscopy (BEM) using a closed bipolar electrode (cBPE) array with an electrochemiluminescence (ECL) detecting system. Because cBPEs are not directly connected to a detector, high spatio-temporal resolution imaging can be achieved by fabricating a microelectrode array in which each electrode point is arranged in a short interval. A cBPE array with individual cBPEs arranged in 41 μm intervals was successfully fabricated by depositing gold in the pores of a track-etched membrane using electroless plating. Using BEM with the cBPE array, which has a higher density of electrode points than the conventional multi-electrode array, we effectively demonstrated the imaging of [Fe(CN)6]3- diffusion and the respiratory activity of MCF-7 spheroids with high spatio-temporal resolution.",

author = "Tomoki Iwama and Inoue, {Kumi Y.} and Hiroya Abe and Tomokazu Matsue and Hitoshi Shiku",

note = "Funding Information: This research was supported by Grant-in-Aid for Scientific Research (C) (no. 19K05687), Grant-in-Aid for Young Scientists (no. 19K15598), and Grant-in-Aid for JSPS Fellows (no. 19J20709) from the Japan Society for the Promotion of Science (JSPS). It was also partially supported by JST COI (no. JPMJCE1303) from the Japan Science and Technology Agency (JST). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

year = "2020",

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doi = "10.1039/d0an00912a",

language = "English",

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AU - Inoue, Kumi Y.

AU - Abe, Hiroya

AU - Matsue, Tomokazu

AU - Shiku, Hitoshi

N1 - Funding Information: This research was supported by Grant-in-Aid for Scientific Research (C) (no. 19K05687), Grant-in-Aid for Young Scientists (no. 19K15598), and Grant-in-Aid for JSPS Fellows (no. 19J20709) from the Japan Society for the Promotion of Science (JSPS). It was also partially supported by JST COI (no. JPMJCE1303) from the Japan Science and Technology Agency (JST). Publisher Copyright: © The Royal Society of Chemistry.

PY - 2020/11/7

Y1 - 2020/11/7

N2 - In this study, we developed bipolar electrochemical microscopy (BEM) using a closed bipolar electrode (cBPE) array with an electrochemiluminescence (ECL) detecting system. Because cBPEs are not directly connected to a detector, high spatio-temporal resolution imaging can be achieved by fabricating a microelectrode array in which each electrode point is arranged in a short interval. A cBPE array with individual cBPEs arranged in 41 μm intervals was successfully fabricated by depositing gold in the pores of a track-etched membrane using electroless plating. Using BEM with the cBPE array, which has a higher density of electrode points than the conventional multi-electrode array, we effectively demonstrated the imaging of [Fe(CN)6]3- diffusion and the respiratory activity of MCF-7 spheroids with high spatio-temporal resolution.

AB - In this study, we developed bipolar electrochemical microscopy (BEM) using a closed bipolar electrode (cBPE) array with an electrochemiluminescence (ECL) detecting system. Because cBPEs are not directly connected to a detector, high spatio-temporal resolution imaging can be achieved by fabricating a microelectrode array in which each electrode point is arranged in a short interval. A cBPE array with individual cBPEs arranged in 41 μm intervals was successfully fabricated by depositing gold in the pores of a track-etched membrane using electroless plating. Using BEM with the cBPE array, which has a higher density of electrode points than the conventional multi-electrode array, we effectively demonstrated the imaging of [Fe(CN)6]3- diffusion and the respiratory activity of MCF-7 spheroids with high spatio-temporal resolution.

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Bioimaging using bipolar electrochemical microscopy with improved spatial resolution

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