@article{27d3348f0b244689afae3194e92dc1b0,
title = "Biomembrane-embedded catalysts for membrane-associated protein labeling on red blood cells",
abstract = "Membrane proteins play crucial roles in fundamental biological processes. Here, we propose a new chemical strategy to label and characterize membrane proteins on cell surface. In this study, 4-dimethylaminopyridine (DMAP) is embedded and concentrated on plasma membrane surface (biomembraneembedded DMAP: BeD), allowing to selectively modify membrane proteins with synthetic probes such as a fluorophore and an affinity tag. We applied this strategy to human red blood cells, which demonstrated that membrane proteins such as PAS-1 and Band-3 were selectively labeled.",
author = "Yuki Yasueda and Tomonori Tamura and Keiko Kuwara and Yousuke Takaoka and Itaru Hamachi",
note = "Funding Information: We thank Junichi Ikenouchi (Kyusyu University) and Masato Umeda (Kyoto University) for helpful discussion. We thank E. Ashihara (Kyoto Pharmaceutical University) for his help in taking blood samples. Y.Y. (26-4745) is supported by Japan Society for the Promotion of Science (JSPS) for doctoral fellowships. This work was funded by the Japan Science and Technology Agency (JST) Core Research for Evolutional Science and Technology (CREST) and by JSPS KAKENHI (15H01637) to I.H. Publisher Copyright: {\textcopyright} 2015 The Chemical Society of Japan.",
year = "2015",
doi = "10.1246/cl.150797",
language = "English",
volume = "44",
pages = "1673--1675",
journal = "Chemistry Letters",
issn = "0366-7022",
publisher = "Chemical Society of Japan",
number = "12",
}