@article{b14165acbec94f158e50af32ae522b52,
title = "BODIPY Catalyzes Proximity-Dependent Histidine Labelling",
abstract = "The novel function of BODIPY, a widely used fluorescent probe, as a catalyst for the chemical labelling of histidine is revealed. Singlet oxygen (1O2) produced by the photosensitizer property of BODIPY oxidises histidine residues, and the histidine oxidised by 1O2 is trapped by the nucleophile 1-methyl-4-arylurazole (MAUra). The simple chemical structure of BODIPY facilitates easier modification compared to other photosensitizer molecules and allows catalysis of protein/peptide-labelling with higher histidine selectivity compared to the ruthenium complex. Owing to the short life of 1O2, the reaction was selectively completed in close proximity with BODIPY. Site-selective functionalization of the target/antibody was successfully achieved using ligand-conjugated BODIPY.",
keywords = "BODIPY, histidine labelling, photosensitizer, proximity labelling, singlet oxygen",
author = "Keita Nakane and Tatsuya Niwa and Michihiko Tsushima and Shusuke Tomoshige and Hideki Taguchi and Hiroyuki Nakamura and Minoru Ishikawa and Shinichi Sato",
note = "Funding Information: This work was partially supported by a research grant from the JST FOREST Program (Grant Number JPMJFR2005, Japan)., Grant‐in‐Aide for Transformative Research Areas (A) Biophysical Chemistry for Material Symbiosis (21H05503 to S. S.), and Grant‐in‐Aid for Scientific Research (B) (19H02848 to S. S.) from MEXT, Japan. K. N. was supported by the SUNBOR SCHOLARSHIP of the Suntory Foundation for Life Sciences. M. T. was supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (JSPS Research Fellowships for Young Scientists, 18J21621). Funding Information: This work was partially supported by a research grant from the JST FOREST Program (Grant Number JPMJFR2005, Japan)., Grant-in-Aide for Transformative Research Areas (A) Biophysical Chemistry for Material Symbiosis (21H05503 to S. S.), and Grant-in-Aid for Scientific Research (B) (19H02848 to S. S.) from MEXT, Japan. K. N. was supported by the SUNBOR SCHOLARSHIP of the Suntory Foundation for Life Sciences. M. T. was supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists (JSPS Research Fellowships for Young Scientists, 18J21621). Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",
year = "2022",
month = may,
day = "6",
doi = "10.1002/cctc.202200077",
language = "English",
volume = "14",
journal = "ChemCatChem",
issn = "1867-3880",
publisher = "Wiley - VCH Verlag GmbH & CO. KGaA",
number = "9",
}