Brain arachidonic acid incorporation is decreased in heart fatty acid binding protein gene-ablated mice

Eric J. Murphy, Yuji Owada, Noriko Kitanaka, Hisatake Kondo, Jan F.C. Glatz

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)


Heart fatty acid binding protein (H-FABP) is expressed in neurons, but its role in brain fatty acid incorporation and metabolism is poorly defined. We examined the effect of H-FABP gene ablation on brain incorporation of arachidonic ([1-14C]20:4n-6) or palmitic ([1-14C]16:0) acid in vivo. Analysis of brain mRNA confirmed gene ablation and demonstrated no compensatory changes in the levels of other FABP mRNA in the gene-ablated mice. In brains from H-FABP gene-ablated mice, the incorporation coefficient for [1-14C]20:4n-6 was reduced 24%, while that for [1-14C]16:0 was unaffected. Within the organic and aqueous fractions, significantly more [1-14C]20:4n-6 was distributed into the aqueous fraction, suggesting a disruption in the metabolic targeting of 20:4n-6 in these mice. There was less incorporation of [1-14C]20:4n-6 into total phospholipids and a marked reduction (51%) in the level of incorporation into the choline glycerophospholipids (ChoGpl). Because FABP can influence steady-state lipid mass, brain individual lipid masses were measured. The brain total phospholipid mass was reduced 17% by gene ablation, ascribed to a 27% and 32% reduction in the masses of ChoGpl and sphingomyelin, respectively. Plasmalogen subclass masses were also reduced, suggesting that H-FABP may augment brain plasmalogen synthesis. In gene-ablated mice, the phosphatidylinositol 20:4n-6 level was reduced 25%, while the proportion of total n-6 fatty acids was reduced in the major phospholipid classes. Thus, these results demonstrate for the first time that H-FABP expression influences brain 20:4n-6 uptake and trafficking as well as steady-state brain lipid levels.

Original languageEnglish
Pages (from-to)6350-6360
Number of pages11
Issue number16
Publication statusPublished - 2005 Apr 26


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