TY - JOUR
T1 - C. elegans slit acts in midline, dorsal-ventral, and anterior-posterior guidance via the SAX-3/Robo receptor
AU - Hao, Joe C.
AU - Yu, Timothy W.
AU - Fujisawa, Kazuko
AU - Culotti, Joseph G.
AU - Gengyo-Ando, Keiko
AU - Mitani, Shohei
AU - Moulder, Gary
AU - Barstead, Robert
AU - Tessier-Lavigne, Marc
AU - Bargmann, Cornelia I.
N1 - Funding Information:
We thank Lucie Yang for assistance in scoring neuronal migrations; Jen Zallen for isolating partial slt-1 cDNAs; Gian Garriga for advice and guidance on cell migration; Jen Zallen and Katja Brose for ideas and useful advice during the course of this work; Frédéric Charron, Roland Friedel, Avraham Yaron, and Yimin Zou for stimulating discussions; Fan Wang and Andy Plump for comments on the manuscript; Tom Kidd and Corey Goodman for sharing results prior to publication; Piali Sengupta and Scott Clark for nematode strains; Andy Fire for GFP vectors; and Shannon Grantner and Hai Nguyen for technical support. J.C.H. is a Howard Hughes Medical Institute predoctoral fellow; T.W.Y. is a MINDS predoctoral fellow; and M.T.L. and C.I.B. are Investigators with the Howard Hughes Medical Institute. This work was funded by the Howard Hughes Medical Institute.
PY - 2001
Y1 - 2001
N2 - Robo receptors interact with ligands of the Slit family. The nematode C. elegans has one Robo receptor (SAX-3) and one Slit protein (SLT-1), which direct ventral axon guidance and guidance at the midline. In larvae, slt-1 expression in dorsal muscles repels axons to promote ventral guidance. SLT-1 acts through the SAX-3 receptor, in parallel with the ventral attractant UNC-6 (Netrin). Removing both UNC-6 and SLT-1 eliminates all ventral guidance information for some axons, revealing an underlying longitudinal guidance pathway. In the embryo, slt-1 is expressed at high levels in anterior epidermis. Embryonic expression of SLT-1 provides anterior-posterior guidance information to migrating CAN neurons. Surprisingly, slt-1 mutants do not exhibit the nerve ring and epithelial defects of sax-3 mutants, suggesting that SAX-3 has both Slit-dependent and Slit-independent functions in development.
AB - Robo receptors interact with ligands of the Slit family. The nematode C. elegans has one Robo receptor (SAX-3) and one Slit protein (SLT-1), which direct ventral axon guidance and guidance at the midline. In larvae, slt-1 expression in dorsal muscles repels axons to promote ventral guidance. SLT-1 acts through the SAX-3 receptor, in parallel with the ventral attractant UNC-6 (Netrin). Removing both UNC-6 and SLT-1 eliminates all ventral guidance information for some axons, revealing an underlying longitudinal guidance pathway. In the embryo, slt-1 is expressed at high levels in anterior epidermis. Embryonic expression of SLT-1 provides anterior-posterior guidance information to migrating CAN neurons. Surprisingly, slt-1 mutants do not exhibit the nerve ring and epithelial defects of sax-3 mutants, suggesting that SAX-3 has both Slit-dependent and Slit-independent functions in development.
UR - http://www.scopus.com/inward/record.url?scp=17944370144&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17944370144&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(01)00448-2
DO - 10.1016/S0896-6273(01)00448-2
M3 - Article
C2 - 11604136
AN - SCOPUS:17944370144
SN - 0896-6273
VL - 32
SP - 25
EP - 38
JO - Neuron
JF - Neuron
IS - 1
ER -