C-terminal domain of human CAP18 antimicrobial peptide induces apoptosis in oral squamous cell carcinoma SAS-H1 cells

Kazuhiko Okumura; Akifumi Itoh; Emiko Isogai; Kimiharu Hirose; Yoichiro Hosokawa; Yoshihiro Abiko; Takatoshi Shibata; Michimasa Hirata; Hiroshi Isogai

doi:10.1016/j.canlet.2004.04.006

C-terminal domain of human CAP18 antimicrobial peptide induces apoptosis in oral squamous cell carcinoma SAS-H1 cells

Kazuhiko Okumura, Akifumi Itoh, Emiko Isogai, Kimiharu Hirose, Yoichiro Hosokawa, Yoshihiro Abiko, Takatoshi Shibata, Michimasa Hirata, Hiroshi Isogai

Research output: Contribution to journal › Article › peer-review

86 Citations (Scopus)

Abstract

Mammalian myeloid and epithelial cells express many antimicrobiotic peptides that contribute to innate host defense against invading microbes. In the present study, a 27-mer peptide of the C-terminal domain (hCAP18 _109-135) and analogs of the antimicrobial peptide human cathelicidin LL-37/human cationic antimicrobial protein 18 (hCAP18) were examined for tumoricidal activity. In vitro results showed that hCAP18_109-135 induced apoptosis in human oral squamous cell carcinoma (OSCC), SAS-H1 cells. The hCAP18_109-135 induced mitochondrial depolarization and apoptosis in SAS-H1 cells, but not in healthy human gingival fibroblasts (HGF) and human keratinocyte line HaCaT cells. Caspases were not activated during hCAP18 _109-135-induced apoptosis in SAS-H1 cells. The results indicate that hCAP18_109-135 may induce caspase-independent apoptosis in OSCC but not in normal cells. hCAP18_109-135 can therefore be a useful anti-tumor therapeutic agent in the treatment of OSCC.

Original language	English
Pages (from-to)	185-194
Number of pages	10
Journal	Cancer Letters
Volume	212
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2004.04.006
Publication status	Published - 2004 Aug 30
Externally published	Yes

Keywords

Apoptosis
C-terminal domain of hCAP18 peptides
Human oral squamos cell carcinoma cells
Hydrophobicity
Mitochondrial membrane potential

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2004.04.006

Cite this

@article{54f23fcab61345f19377832fcc43c0bd,

title = "C-terminal domain of human CAP18 antimicrobial peptide induces apoptosis in oral squamous cell carcinoma SAS-H1 cells",

abstract = "Mammalian myeloid and epithelial cells express many antimicrobiotic peptides that contribute to innate host defense against invading microbes. In the present study, a 27-mer peptide of the C-terminal domain (hCAP18 109-135) and analogs of the antimicrobial peptide human cathelicidin LL-37/human cationic antimicrobial protein 18 (hCAP18) were examined for tumoricidal activity. In vitro results showed that hCAP18109-135 induced apoptosis in human oral squamous cell carcinoma (OSCC), SAS-H1 cells. The hCAP18109-135 induced mitochondrial depolarization and apoptosis in SAS-H1 cells, but not in healthy human gingival fibroblasts (HGF) and human keratinocyte line HaCaT cells. Caspases were not activated during hCAP18 109-135-induced apoptosis in SAS-H1 cells. The results indicate that hCAP18109-135 may induce caspase-independent apoptosis in OSCC but not in normal cells. hCAP18109-135 can therefore be a useful anti-tumor therapeutic agent in the treatment of OSCC.",

keywords = "Apoptosis, C-terminal domain of hCAP18 peptides, Human oral squamos cell carcinoma cells, Hydrophobicity, Mitochondrial membrane potential",

author = "Kazuhiko Okumura and Akifumi Itoh and Emiko Isogai and Kimiharu Hirose and Yoichiro Hosokawa and Yoshihiro Abiko and Takatoshi Shibata and Michimasa Hirata and Hiroshi Isogai",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (C) (2), #14571906 from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

year = "2004",

month = aug,

day = "30",

doi = "10.1016/j.canlet.2004.04.006",

language = "English",

volume = "212",

pages = "185--194",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - C-terminal domain of human CAP18 antimicrobial peptide induces apoptosis in oral squamous cell carcinoma SAS-H1 cells

AU - Okumura, Kazuhiko

AU - Itoh, Akifumi

AU - Isogai, Emiko

AU - Hirose, Kimiharu

AU - Hosokawa, Yoichiro

AU - Abiko, Yoshihiro

AU - Shibata, Takatoshi

AU - Hirata, Michimasa

AU - Isogai, Hiroshi

N1 - Funding Information: This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (C) (2), #14571906 from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2004/8/30

Y1 - 2004/8/30

N2 - Mammalian myeloid and epithelial cells express many antimicrobiotic peptides that contribute to innate host defense against invading microbes. In the present study, a 27-mer peptide of the C-terminal domain (hCAP18 109-135) and analogs of the antimicrobial peptide human cathelicidin LL-37/human cationic antimicrobial protein 18 (hCAP18) were examined for tumoricidal activity. In vitro results showed that hCAP18109-135 induced apoptosis in human oral squamous cell carcinoma (OSCC), SAS-H1 cells. The hCAP18109-135 induced mitochondrial depolarization and apoptosis in SAS-H1 cells, but not in healthy human gingival fibroblasts (HGF) and human keratinocyte line HaCaT cells. Caspases were not activated during hCAP18 109-135-induced apoptosis in SAS-H1 cells. The results indicate that hCAP18109-135 may induce caspase-independent apoptosis in OSCC but not in normal cells. hCAP18109-135 can therefore be a useful anti-tumor therapeutic agent in the treatment of OSCC.

AB - Mammalian myeloid and epithelial cells express many antimicrobiotic peptides that contribute to innate host defense against invading microbes. In the present study, a 27-mer peptide of the C-terminal domain (hCAP18 109-135) and analogs of the antimicrobial peptide human cathelicidin LL-37/human cationic antimicrobial protein 18 (hCAP18) were examined for tumoricidal activity. In vitro results showed that hCAP18109-135 induced apoptosis in human oral squamous cell carcinoma (OSCC), SAS-H1 cells. The hCAP18109-135 induced mitochondrial depolarization and apoptosis in SAS-H1 cells, but not in healthy human gingival fibroblasts (HGF) and human keratinocyte line HaCaT cells. Caspases were not activated during hCAP18 109-135-induced apoptosis in SAS-H1 cells. The results indicate that hCAP18109-135 may induce caspase-independent apoptosis in OSCC but not in normal cells. hCAP18109-135 can therefore be a useful anti-tumor therapeutic agent in the treatment of OSCC.

KW - Apoptosis

KW - C-terminal domain of hCAP18 peptides

KW - Human oral squamos cell carcinoma cells

KW - Hydrophobicity

KW - Mitochondrial membrane potential

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JF - Cancer Letters

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C-terminal domain of human CAP18 antimicrobial peptide induces apoptosis in oral squamous cell carcinoma SAS-H1 cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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