Caenorhabditis elegans Chk2-like gene is essential for meiosis but dispensable for DNA repair

Atsushi Higashitani, Hidetoshi Aoki, Akiyuki Mori, Yohei Sasagawa, Takako Takanami, Hideyuki Takahashi

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


A Chk2-like gene was identified in the genome of Caenorhabditis elegans. The putative gene product, termed Ce-chk-2 consists of 450 amino acid residues, and shows good homology with the Chk2/Cds1 gene family. The results of RNA-mediated interference (RNAi) indicated that the F1 generation from dsRNA injected animals grew to adulthood, but approximately 95% of their eggs (F2) died during early embryogenesis. Among the few surviving progeny, males (XO animals) arose at an abnormally high frequency (30%). In addition, 12 univalents were observed in full grown oocytes of the F1, while six bivalents were normally observed in wild-type oocytes. Ce-chk-2 gene expression increased in the adult stage, and their expression level decreased in the glp-4 mutant, which is defective in germ line proliferation. The radiation sensitivity of F1 embryos carrying Ce-chk-2 RNAi was not significantly affected. (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalFEBS Letters
Issue number1
Publication statusPublished - 2000 Nov 17


  • Chiasma
  • Chk2/Cds1
  • Forkhead homology-associated domain
  • Pairing
  • Protein kinase
  • RNA-mediated interference


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