TY - JOUR
T1 - Caenorhabditis elegans reticulon interacts with RME-1 during embryogenesis
AU - Iwahashi, Jun
AU - Kawasaki, Ichiro
AU - Kohara, Yuji
AU - Gengyo-Ando, Keiko
AU - Mitani, Shohei
AU - Ohshima, Yasumi
AU - Hamada, Nobuyuki
AU - Hara, Koyu
AU - Kashiwagi, Takahito
AU - Toyoda, Tetsuya
N1 - Funding Information:
We thank Dr. A. Coulson for cosmid W06A7, Dr. A. Fire for plasmid pPD95.67, Dr. R. Barstead for a mixed-stage C. elegans cDNA library, and Dr. H.R. Horvitz for ced-9 cDNA. This study was partly supported by the Sasakawa Scientific Research Grant from the Japan Science Society.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Reticulon (RTN) family proteins are localized in the endoplasmic reticulum (ER). At least four different RTN genes have been identified in mammals, but in most cases, the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are unknown. Each RTN gene produces 1-3 proteins by different promoters and alternative splicing. In Caenorhabditis elegans, there is a single gene (rtn gene) encoding three reticulon proteins, nRTN-A, B, and C. mRNA of nRTN-C is expressed in germ cells and embryos. However, nRTN-C protein is only expressed during embryogenesis and rapidly disappears after hatch. By yeast two-hybrid screening, two clones encoding the same C-terminal region of RME-1, a protein functioning in the endocytic recycling, were isolated. These findings suggest that nRTN-C functions in the endocytic pathway during embryogenesis.
AB - Reticulon (RTN) family proteins are localized in the endoplasmic reticulum (ER). At least four different RTN genes have been identified in mammals, but in most cases, the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are unknown. Each RTN gene produces 1-3 proteins by different promoters and alternative splicing. In Caenorhabditis elegans, there is a single gene (rtn gene) encoding three reticulon proteins, nRTN-A, B, and C. mRNA of nRTN-C is expressed in germ cells and embryos. However, nRTN-C protein is only expressed during embryogenesis and rapidly disappears after hatch. By yeast two-hybrid screening, two clones encoding the same C-terminal region of RME-1, a protein functioning in the endocytic recycling, were isolated. These findings suggest that nRTN-C functions in the endocytic pathway during embryogenesis.
KW - C. elegans
KW - Endocytosis
KW - Neuroendocrine specific protein
KW - Reticulon
KW - RME-1
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U2 - 10.1016/S0006-291X(02)00282-6
DO - 10.1016/S0006-291X(02)00282-6
M3 - Article
C2 - 12054525
AN - SCOPUS:0036297098
SN - 0006-291X
VL - 293
SP - 698
EP - 704
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -