Caffeine-resistance in fission yeast is caused by mutations in a single essential gene, crm1+

Kazuki Kumada, Mitsuhiro Yanagida, Takashi Toda

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47 Citations (Scopus)


Caffeine is a base analogue and is known to affect a wide variety of cellular processes. In order to dissect genetically molecules which mediate the biological effects of caffeine, temperature-sensitive (ts) and caffeine-resistant mutants were isolated from fission yeast, Schizosaccharomyces pombe. Surprisingly, all twelve ts isolates contained a mutation in the same locus, crm1. Cells of the ts crml mutant showed an abnormal chromosome structure at the restrictive temperature, an elevated expression of Pap1-dependent transcription, and cross-resistance to an unrelated drug such as staurosporine. Overproduction of pap1+ also conferred caffeine resistance, whilst the resistance of the crm1 mutant is abolished in the papl- background. These results show that the crml+ gene is a major locus for caffeine resistance, which arises from Pap1-dependent transcriptional activation.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalMolecular and General Genetics
Issue number1
Publication statusPublished - 1996


  • Caffeine
  • Chromosome structure
  • Fission yeast
  • Multidrug resistance
  • Transcription factor


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