Calcitonin gene-related peptide-like immunoreactivities in pheochromocytomas

Toraichi Mouri; Kazuhiro Takahashi; Masahiko Sone; Osamu Murakami; Makoto Ohneda; Keiichi Itoi; Yutaka Imai; Kaoru Yoshinaga; Nobuaki Sasano

doi:10.1016/0196-9781(89)90097-1

Calcitonin gene-related peptide-like immunoreactivities in pheochromocytomas

Toraichi Mouri, Kazuhiro Takahashi, Masahiko Sone, Osamu Murakami, Makoto Ohneda, Keiichi Itoi, Yutaka Imai, Kaoru Yoshinaga, Nobuaki Sasano

MED - Health Sciences

Tohoku University

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Calcitonin gene-related peptide (CGRP) is reported to exist in high concentrations in plasma and tumor tissues of medullary thyroid carcinomas. CGRP-like immunoreactivity (LI) in tumor tissues of pheochromocytomas was investigated by radioimmunoassay. CGRP-LI in 9 pheochromocytomas ranged from 0.50 to 1240 ng/g wet tissue. Sephadex G-50 column chromatography revealed that most of CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP. Reverse-phase high pressure liquid chromatography revealed that CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP and in a more hydrophobic position. These results indicate that high concentrations of CGRP-LI also exist in tumor tissues of pheochromocytomas.

Original language	English
Pages (from-to)	201-204
Number of pages	4
Journal	Peptides
Volume	10
Issue number	1
DOIs	https://doi.org/10.1016/0196-9781(89)90097-1
Publication status	Published - 1989

Keywords

Calcitonin gene-related peptide (CGRP)
Pheochromocytoma
Radioimmunoassay

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10.1016/0196-9781(89)90097-1

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title = "Calcitonin gene-related peptide-like immunoreactivities in pheochromocytomas",

abstract = "Calcitonin gene-related peptide (CGRP) is reported to exist in high concentrations in plasma and tumor tissues of medullary thyroid carcinomas. CGRP-like immunoreactivity (LI) in tumor tissues of pheochromocytomas was investigated by radioimmunoassay. CGRP-LI in 9 pheochromocytomas ranged from 0.50 to 1240 ng/g wet tissue. Sephadex G-50 column chromatography revealed that most of CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP. Reverse-phase high pressure liquid chromatography revealed that CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP and in a more hydrophobic position. These results indicate that high concentrations of CGRP-LI also exist in tumor tissues of pheochromocytomas.",

keywords = "Calcitonin gene-related peptide (CGRP), Pheochromocytoma, Radioimmunoassay",

author = "Toraichi Mouri and Kazuhiro Takahashi and Masahiko Sone and Osamu Murakami and Makoto Ohneda and Keiichi Itoi and Yutaka Imai and Kaoru Yoshinaga and Nobuaki Sasano",

note = "Funding Information: This study has been supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan (No. 615702958). We thank Miss Toshie Katayanagi for her secretarial assistance.",

year = "1989",

doi = "10.1016/0196-9781(89)90097-1",

language = "English",

volume = "10",

pages = "201--204",

journal = "Peptides",

issn = "0196-9781",

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T1 - Calcitonin gene-related peptide-like immunoreactivities in pheochromocytomas

AU - Mouri, Toraichi

AU - Takahashi, Kazuhiro

AU - Sone, Masahiko

AU - Murakami, Osamu

AU - Ohneda, Makoto

AU - Itoi, Keiichi

AU - Imai, Yutaka

AU - Yoshinaga, Kaoru

AU - Sasano, Nobuaki

N1 - Funding Information: This study has been supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan (No. 615702958). We thank Miss Toshie Katayanagi for her secretarial assistance.

PY - 1989

Y1 - 1989

N2 - Calcitonin gene-related peptide (CGRP) is reported to exist in high concentrations in plasma and tumor tissues of medullary thyroid carcinomas. CGRP-like immunoreactivity (LI) in tumor tissues of pheochromocytomas was investigated by radioimmunoassay. CGRP-LI in 9 pheochromocytomas ranged from 0.50 to 1240 ng/g wet tissue. Sephadex G-50 column chromatography revealed that most of CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP. Reverse-phase high pressure liquid chromatography revealed that CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP and in a more hydrophobic position. These results indicate that high concentrations of CGRP-LI also exist in tumor tissues of pheochromocytomas.

AB - Calcitonin gene-related peptide (CGRP) is reported to exist in high concentrations in plasma and tumor tissues of medullary thyroid carcinomas. CGRP-like immunoreactivity (LI) in tumor tissues of pheochromocytomas was investigated by radioimmunoassay. CGRP-LI in 9 pheochromocytomas ranged from 0.50 to 1240 ng/g wet tissue. Sephadex G-50 column chromatography revealed that most of CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP. Reverse-phase high pressure liquid chromatography revealed that CGRP-LI in tumor extracts was eluted in an identical position to synthetic human CGRP and in a more hydrophobic position. These results indicate that high concentrations of CGRP-LI also exist in tumor tissues of pheochromocytomas.

KW - Calcitonin gene-related peptide (CGRP)

KW - Pheochromocytoma

KW - Radioimmunoassay

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AN - SCOPUS:0024564471

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VL - 10

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JO - Peptides

JF - Peptides

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ER -

Calcitonin gene-related peptide-like immunoreactivities in pheochromocytomas

Abstract

Keywords

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