TY - JOUR
T1 - Canonical Wnt signaling and its antagonist regulate anterior-posterior axis polarization by guiding cell migration in mouse visceral endoderm
AU - Kimura-Yoshida, Chiharu
AU - Nakano, Hiroshi
AU - Okamura, Daiji
AU - Nakao, Kazuki
AU - Yonemura, Shigenobu
AU - Belo, Jose A.
AU - Aizawa, Shinichi
AU - Matsui, Yasuhisa
AU - Matsuo, Isao
N1 - Funding Information:
We are grateful to Drs. E. De Robertis, R. Grosschedl, B.G. Herrmann, B. Hogan, G. Martin, R. Nusse, M.M. Shen, and K. Yamamura for in situ probes. We also wish to thank Dr. C. Niehrs for Dkk1 cDNA plasmid; the Animal Resources and Genetic Engineering Team, RIKEN Center for Developmental Biology for housing the mice; and Ms. Naoko Inoue, Mr. Hiroshi Kiyonari, Ms. Rika Nakayama, Ms. Ayako Nagao, and Ms. Kuniko Kitajima for their assistance. This work was supported in part by grants-in-aid for Scientific Research on Priority Areas and Young Scientists (B) from the Ministry of Education, Culture, Sports Science and Technology, Japan.
PY - 2005/11
Y1 - 2005/11
N2 - The mouse embryonic axis is initially formed with a proximal-distal orientation followed by subsequent conversion to a prospective anterior-posterior (A-P) polarity with directional migration of visceral endoderm cells. Importantly, Otx2, a homeobox gene, is essential to this developmental process. However, the genetic regulatory mechanism governing axis conversion is poorly understood. Here, defective axis conversion due to Otx2 deficiency can be rescued by expression of Dkk1, a Wnt antagonist, or following removal of one copy of the β-catenin gene. Misexpression of a canonical Wnt ligand can also inhibit correct A-P axis rotation. Moreover, asymmetrical distribution of β-catenin localization is impaired in the Otx2-deficient and Wnt-misexpressing visceral endoderm. Concurrently, canonical Wnt and Dkk1 function as repulsive and attractive guidance cues, respectively, in the migration of visceral endoderm cells. We propose that Wnt/β-catenin signaling mediates A-P axis polarization by guiding cell migration toward the prospective anterior in the pregastrula mouse embryo.
AB - The mouse embryonic axis is initially formed with a proximal-distal orientation followed by subsequent conversion to a prospective anterior-posterior (A-P) polarity with directional migration of visceral endoderm cells. Importantly, Otx2, a homeobox gene, is essential to this developmental process. However, the genetic regulatory mechanism governing axis conversion is poorly understood. Here, defective axis conversion due to Otx2 deficiency can be rescued by expression of Dkk1, a Wnt antagonist, or following removal of one copy of the β-catenin gene. Misexpression of a canonical Wnt ligand can also inhibit correct A-P axis rotation. Moreover, asymmetrical distribution of β-catenin localization is impaired in the Otx2-deficient and Wnt-misexpressing visceral endoderm. Concurrently, canonical Wnt and Dkk1 function as repulsive and attractive guidance cues, respectively, in the migration of visceral endoderm cells. We propose that Wnt/β-catenin signaling mediates A-P axis polarization by guiding cell migration toward the prospective anterior in the pregastrula mouse embryo.
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U2 - 10.1016/j.devcel.2005.09.011
DO - 10.1016/j.devcel.2005.09.011
M3 - Article
C2 - 16256739
AN - SCOPUS:27644590039
SN - 1534-5807
VL - 9
SP - 639
EP - 650
JO - Developmental Cell
JF - Developmental Cell
IS - 5
ER -