Carbon-Ag/AgCl probes for detection of cell activity in droplets

Kosuke Ino; Kaoru Ono; Toshiharu Arai; Yasufumi Takahashi; Hitoshi Shiku; Tomokazu Matsue

doi:10.1021/ac303569t

Carbon-Ag/AgCl probes for detection of cell activity in droplets

Kosuke Ino, Kaoru Ono, Toshiharu Arai, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

Tohoku University

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.

Original language	English
Pages (from-to)	3832-3835
Number of pages	4
Journal	Analytical Chemistry
Volume	85
Issue number	8
DOIs	https://doi.org/10.1021/ac303569t
Publication status	Published - 2013 Apr 16

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abstract = "In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.",

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AU - Ino, Kosuke

AU - Ono, Kaoru

AU - Arai, Toshiharu

AU - Takahashi, Yasufumi

AU - Shiku, Hitoshi

AU - Matsue, Tomokazu

PY - 2013/4/16

Y1 - 2013/4/16

N2 - In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.

AB - In this study, we fabricated a probe consisting of a carbon nanoelectrode and an Ag/AgCl reference electrode for detecting the activity of cells in single droplets. HeLa cells were confined into a single droplet, and the alkaline phosphatase (ALP) activity of the cells was electrochemically measured using the probe inserted into the droplet. The ALP of the confined cells catalyzed the hydrolysis of p-aminophenyl phosphate (PAPP) to yield p-aminophenol (PAP) that gave electrochemical responses. Since the tip of the carbon-Ag/AgCl probe is very small, it is useful for electrochemical analysis of cells using droplets.

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Carbon-Ag/AgCl probes for detection of cell activity in droplets

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