TY - JOUR
T1 - Carbon nanotubes embedded in embryoid bodies direct cardiac differentiation
AU - Ahadian, Samad
AU - Yamada, Shukuyo
AU - Estili, Mehdi
AU - Liang, Xiaobin
AU - Banan Sadeghian, Ramin
AU - Nakajima, Ken
AU - Shiku, Hitoshi
AU - Matsue, Tomokazu
AU - Khademhosseini, Ali
N1 - Funding Information:
This work was supported by the World Premier International Research Center Initiative (WPI), MEXT, Japan.
Publisher Copyright:
© 2017, Springer Science+Business Media New York.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - We embedded carbon nanotubes (CNTs) in mouse embryoid bodies (EBs) for modulating mechanical and electrical cues of the stem cell niche. The CNTs increased the mechanical integrity and electrical conductivity of the EBs. Measured currents for the unmodified EBs (hereafter, EBs) and the EBs-0.25 mg/mL CNTs were 0.79 and 26.3 mA, respectively, at voltage of 5 V. The EBs had a Young’s modulus of 20.9 ± 6.5 kPa, whereas the Young’s modulus of the EB-0.1 mg/mL CNTs was 35.2 ± 5.6 kPa. The EB-CNTs also showed lower proliferation and greater differentiation rates compared with the EBs as determined by the expression of pluripotency genes and the analysis of EB sizes. Interestingly, the cardiac differentiation of the EB-CNTs was significantly greater than that of the EBs, as confirmed by high-throughput gene analysis at day 5 of culture. Applying electrical stimulation to the EB-CNTs specifically enhanced the cardiac differentiation and beating activity of the EBs.
AB - We embedded carbon nanotubes (CNTs) in mouse embryoid bodies (EBs) for modulating mechanical and electrical cues of the stem cell niche. The CNTs increased the mechanical integrity and electrical conductivity of the EBs. Measured currents for the unmodified EBs (hereafter, EBs) and the EBs-0.25 mg/mL CNTs were 0.79 and 26.3 mA, respectively, at voltage of 5 V. The EBs had a Young’s modulus of 20.9 ± 6.5 kPa, whereas the Young’s modulus of the EB-0.1 mg/mL CNTs was 35.2 ± 5.6 kPa. The EB-CNTs also showed lower proliferation and greater differentiation rates compared with the EBs as determined by the expression of pluripotency genes and the analysis of EB sizes. Interestingly, the cardiac differentiation of the EB-CNTs was significantly greater than that of the EBs, as confirmed by high-throughput gene analysis at day 5 of culture. Applying electrical stimulation to the EB-CNTs specifically enhanced the cardiac differentiation and beating activity of the EBs.
KW - Carbon nanotubes
KW - Cardiac differentiation
KW - Electrical stimulation
KW - Mouse embryoid body
KW - Proliferation
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U2 - 10.1007/s10544-017-0184-1
DO - 10.1007/s10544-017-0184-1
M3 - Article
C2 - 28634847
AN - SCOPUS:85021180381
SN - 1387-2176
VL - 19
JO - Biomedical Microdevices
JF - Biomedical Microdevices
IS - 3
M1 - 57
ER -