Catalyst-proximity protein chemical labelling on affinity beads targeting endogenous lectins

Michihiko Tsushima, Shinichi Sato, Tatsuya Niwa, Hideki Taguchi, Hiroyuki Nakamura

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Magnetic affinity beads functionalized with lactose and ruthenium/dcbpy complexes were developed. Using MAUra, a catalyst-proximity labelling reagent, the catalytic labeling of lactose-binding proteins was achieved with high selectivity on the beads. The first unbiased identification of cellular endogenous lectins bound to lactose (galectin-1 and galectin-3) was achieved with chemical labelling on the affinity beads.

Original languageEnglish
Pages (from-to)13275-13278
Number of pages4
JournalChemical Communications
Volume55
Issue number88
DOIs
Publication statusPublished - 2019

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