Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide

Yusuke Hatakawa; Rina Nakamura; Motomi Konishi; Toshiyasu Sakane; Motoaki Saito; Toshifumi Akizawa

doi:10.1016/j.heliyon.2019.e02454

Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide

Yusuke Hatakawa, Rina Nakamura, Motomi Konishi, Toshiyasu Sakane, Motoaki Saito, Toshifumi Akizawa

PHA - Life and Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of ANA-TA9 corresponding region to JAL-TA9 in ANA/BTG3 protein. ANA-TA9 showed the auto-proteolytic activity and cleaved 3 kinds of synthetic fragment peptides derived from Aβ42, especially on the central region of Aβ42 with a serine protease like activity. Interestingly, 2 kinds of components, ANA-SA5 (SKGQA) and ANA-YA4 (YRMI), also showed similar proteolytic activity. These results indicate that ANA-TA9 is composed of two different Catalytides.

Original language	English
Article number	e02454
Journal	Heliyon
Volume	5
Issue number	9
DOIs	https://doi.org/10.1016/j.heliyon.2019.e02454
Publication status	Published - 2019 Sept

Keywords

Alzheimer's disease
Amyloid-β peptide
ANA/BTG3
Analytical chemistry
Biochemistry
Biophysical chemistry
Biotechnology
Box A region
Catalytide
Chromatography
Developmental biology
Hydrolase activity
Molecular biology
Molecular modeling
Peptides
Structural biology
Tob/BTG

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title = "Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide",

abstract = "We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of ANA-TA9 corresponding region to JAL-TA9 in ANA/BTG3 protein. ANA-TA9 showed the auto-proteolytic activity and cleaved 3 kinds of synthetic fragment peptides derived from Aβ42, especially on the central region of Aβ42 with a serine protease like activity. Interestingly, 2 kinds of components, ANA-SA5 (SKGQA) and ANA-YA4 (YRMI), also showed similar proteolytic activity. These results indicate that ANA-TA9 is composed of two different Catalytides.",

keywords = "Alzheimer's disease, Amyloid-β peptide, ANA/BTG3, Analytical chemistry, Biochemistry, Biophysical chemistry, Biotechnology, Box A region, Catalytide, Chromatography, Developmental biology, Hydrolase activity, Molecular biology, Molecular modeling, Peptides, Structural biology, Tob/BTG",

author = "Yusuke Hatakawa and Rina Nakamura and Motomi Konishi and Toshiyasu Sakane and Motoaki Saito and Toshifumi Akizawa",

note = "Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) Program, Grant Number 15K07908 , and the Okinawa Institute of Science and Technology (OIST) Proof-of Concept (POC) Program. Publisher Copyright: {\textcopyright} 2019 The Author(s)",

year = "2019",

month = sep,

doi = "10.1016/j.heliyon.2019.e02454",

language = "English",

volume = "5",

journal = "Heliyon",

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T1 - Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide

AU - Hatakawa, Yusuke

AU - Nakamura, Rina

AU - Konishi, Motomi

AU - Sakane, Toshiyasu

AU - Saito, Motoaki

AU - Akizawa, Toshifumi

N1 - Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) Program, Grant Number 15K07908 , and the Okinawa Institute of Science and Technology (OIST) Proof-of Concept (POC) Program. Publisher Copyright: © 2019 The Author(s)

PY - 2019/9

Y1 - 2019/9

N2 - We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of ANA-TA9 corresponding region to JAL-TA9 in ANA/BTG3 protein. ANA-TA9 showed the auto-proteolytic activity and cleaved 3 kinds of synthetic fragment peptides derived from Aβ42, especially on the central region of Aβ42 with a serine protease like activity. Interestingly, 2 kinds of components, ANA-SA5 (SKGQA) and ANA-YA4 (YRMI), also showed similar proteolytic activity. These results indicate that ANA-TA9 is composed of two different Catalytides.

AB - We have recently reported about shorter proteolytic peptides termed Catalytide as general name. JAL-TA9 (YKGSGFRMI), a fragment peptide derived from Box A region of Tob1 protein, is the first Catalytide and cleaves Aβ42 and its fragment peptides. Herein, we demonstrate the enzymatic properties of ANA-TA9 corresponding region to JAL-TA9 in ANA/BTG3 protein. ANA-TA9 showed the auto-proteolytic activity and cleaved 3 kinds of synthetic fragment peptides derived from Aβ42, especially on the central region of Aβ42 with a serine protease like activity. Interestingly, 2 kinds of components, ANA-SA5 (SKGQA) and ANA-YA4 (YRMI), also showed similar proteolytic activity. These results indicate that ANA-TA9 is composed of two different Catalytides.

KW - Alzheimer's disease

KW - Amyloid-β peptide

KW - ANA/BTG3

KW - Analytical chemistry

KW - Biochemistry

KW - Biophysical chemistry

KW - Biotechnology

KW - Box A region

KW - Catalytide

KW - Chromatography

KW - Developmental biology

KW - Hydrolase activity

KW - Molecular biology

KW - Molecular modeling

KW - Peptides

KW - Structural biology

KW - Tob/BTG

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DO - 10.1016/j.heliyon.2019.e02454

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SN - 2405-8440

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JO - Heliyon

JF - Heliyon

IS - 9

M1 - e02454

ER -

Catalytides derived from the Box A region in the ANA/BTG3 protein cleave amyloid-β fragment peptide

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