cDNA cloning of mitochondrial Δ3, Δ2-enoyl-CoA isomerase of rat liver

Yoshihisa Tomioka, Akihiko Hirose, Hiroshi Moritani, Takanori Hishinuma, Takashi Hashimoto, Michinao Mizugaki

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


A 1.08 kbp cDNA encoding rat liver mitochondrial Δ3, Δ2-enoyl-CoA isomerase (ECI) of 298 amino acid residues (Mr 32 895) was isolated from rat liver λgt11, λgt10 cDNA libraries by the combination of an immunochemical method with a rabbit-antibody against rat liver ECI and a plaque hybridization method. The deduced amino acid sequence from the cDNA indicates that ECI is synthesized with an amino-terminal extrasequence of 35 amino acid residues and processed to the mature enzyme (Mr 29 256).

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalBBA - Gene Structure and Expression
Issue number1
Publication statusPublished - 1992 Feb 28


  • (Rat liver)
  • cDNA
  • β-Oxidation
  • Δ, Δ-Enoyl-CoA isomerase
  • λgt10
  • λgt11


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