TY - JOUR
T1 - Cell activity on Type 316L stainless steel with self-organized nanopores formed by anodic polarization
AU - Miyabe, Sayaka
AU - Suehiro, Takashi
AU - Fujinaga, Yushi
AU - Tsuchiya, Hiroaki
AU - Hiromoto, Sachiko
AU - Fujimoto, Shinji
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (A) (project nos. 23246128 and 26249111) and Grant-in-Aid for Young Scientists (B) (project no. 25870410) from the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2016 The Japan Institute of Metals and Materials.
PY - 2016
Y1 - 2016
N2 - In this study, we evaluated the osseoconductivity of Type 316L stainless steel with self-organized nanopores of three different average diameters (26, 90, and 177 nm), formed by anodic polarization. The proliferation, alkaline phosphatase activity, and morphology of MC3T3-E1 mouse osteoblast-like cells, cultured on the self-organized nanopores were evaluated. The cell densities on samples with the nanopores were higher than those on mechanically finished surfaces that were mirror-polished or ground with # 2000 SiC paper. In particular, the highest cell density and alkaline phosphatase activity were obtained on the nanoporous sample with the smallest diameter of 26 nm. Cells on the samples with 26 nm nanopores extended further and spread more filopodia compared with cells on samples with the other surface morphologies. Therefore, we concluded that self-organized nanopores with an optimal diameter (e.g., 26 nm) on Type 316L stainless steel could enhance long-term cell activity.
AB - In this study, we evaluated the osseoconductivity of Type 316L stainless steel with self-organized nanopores of three different average diameters (26, 90, and 177 nm), formed by anodic polarization. The proliferation, alkaline phosphatase activity, and morphology of MC3T3-E1 mouse osteoblast-like cells, cultured on the self-organized nanopores were evaluated. The cell densities on samples with the nanopores were higher than those on mechanically finished surfaces that were mirror-polished or ground with # 2000 SiC paper. In particular, the highest cell density and alkaline phosphatase activity were obtained on the nanoporous sample with the smallest diameter of 26 nm. Cells on the samples with 26 nm nanopores extended further and spread more filopodia compared with cells on samples with the other surface morphologies. Therefore, we concluded that self-organized nanopores with an optimal diameter (e.g., 26 nm) on Type 316L stainless steel could enhance long-term cell activity.
KW - Anodic polarization
KW - Biomaterials
KW - Nanopores
KW - Osseoconductivity
KW - Osteoblast
KW - Type 316L stainless steels
UR - http://www.scopus.com/inward/record.url?scp=84998611008&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84998611008&partnerID=8YFLogxK
U2 - 10.2320/matertrans.MI201516
DO - 10.2320/matertrans.MI201516
M3 - Article
AN - SCOPUS:84998611008
SN - 1345-9678
VL - 57
SP - 2065
EP - 2071
JO - Materials Transactions
JF - Materials Transactions
IS - 12
ER -