Cell scooper: A device for the rapid transfer of living cell sheet

Kenjiro Tadakuma, Nobuyuki Tanaka, Yuji Haraguchi, Mitsuru Higashimori, Makoto Kaneko, Tatsuya Shimizu, Masayuki Yamato, Teruo Okano

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


In this study, we developed a device that could easily, rapidly, and completely transfer cell sheets from one material to another or transplant cell sheets onto the dorsal subcutaneous tissues of rats without leaving residual cells. Because the manipulation is as simple as pipetting, technical expertise is not required to transfer cell sheets very rapidly (the transfer time was 3.7 ± 1.6 s) using the device compared with that of a conventional method using a pipette (430 ± 180 s). After transfer by the device, C2C12 skeletal myoblast sheets showed active cell metabolism, cell viability, and very high production of vascular endothelial growth factor and stromal-derived factor-1α, indicating transfer without cell damage. Cardiac cell sheets after transfer showed spontaneous and synchronous beating, indicating intact cellcell junctions and ion channel proteins on the cell opsurface. In addition, the device allowed us to transfer C2C12 cell sheets onto soft, rugged and curved surfaces such as human hands. Furthermore, cardiac cell sheets adhered rapidly and tightly onto the dorsal subcutaneous tissues of rats. This transfer/transplantation device may be a powerful tool in cell sheet-based tissue engineering and regenerative medicine.

Original languageEnglish
Title of host publicationHyper Bio Assembler for 3D Cellular Systems
PublisherSpringer Japan
Number of pages14
ISBN (Electronic)9784431552970
ISBN (Print)9784431552963
Publication statusPublished - 2015 Jan 1


  • Device
  • Mechanism
  • Scooping
  • Transfer


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