@article{261d369eb7b94f00ae06e9914629bc1f,
title = "Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium",
abstract = "Transplantation of hiPSC-derived corneal epithelial cell (iCEC) sheets is expected to serve as a treatment for severe corneal diseases. The efficacy of this treatment depends on effective iCEC purification. Hayashi and colleagues used differential laminin adhesion of iCECs and non-CECs in combination magnetic cell sorting to develop a simple method to obtain highly pure iCEC sheets without using FACS.",
keywords = "cell competition, cell purification, corneal epithelial cells (CECs), human induced pluripotent stem cells (hiPSCs), laminin isoforms, substrate-specific adhesion",
author = "Shun Shibata and Ryuhei Hayashi and Yuji Kudo and Toru Okubo and Tsutomu Imaizumi and Tomohiko Katayama and Yuki Ishikawa and Yuki Kobayashi and Junko Toga and Yukimasa Taniguchi and Yoichi Honma and Kiyotoshi Sekiguchi and Kohji Nishida",
note = "Funding Information: We thank S. Hara, H. Takayanagi, Y. Yasukawa, Y. Yamate, S. Azuma, K. Suzuki, Y. Ogawa, and M. Morita of Osaka University for their technical assistance. This work was supported in part by the project for the realization of regenerative medicine of the Japan Agency for Medical Research and Development (AMED) and Grant-in-Aid for Scientific Research (C) ( 17K11480 ) from the Japan Society for the Promotion of Science (JSPS). S.S., Y. Kudo, T.O., T.I., and Y.H. are employees of ROHTO Pharmaceutical Co., Ltd. R.H. is affiliated with the endowed chair of ROHTO Pharmaceutical Co., Ltd. K.S. is a co-founder and shareholder of Matrixome, Inc. Y.T. holds a position of Project Leader at Matrixome, Inc. T.K., Y.I., Y.K., J.T., and K.N. have no competing interests. Funding Information: We thank S. Hara, H. Takayanagi, Y. Yasukawa, Y. Yamate, S. Azuma, K. Suzuki, Y. Ogawa, and M. Morita of Osaka University for their technical assistance. This work was supported in part by the project for the realization of regenerative medicine of the Japan Agency for Medical Research and Development (AMED) and Grant-in-Aid for Scientific Research (C) (17K11480) from the Japan Society for the Promotion of Science (JSPS). S.S. Y. Kudo, T.O. T.I. and Y.H. are employees of ROHTO Pharmaceutical Co. Ltd. R.H. is affiliated with the endowed chair of ROHTO Pharmaceutical Co. Ltd. K.S. is a co-founder and shareholder of Matrixome, Inc. Y.T. holds a position of Project Leader at Matrixome, Inc. T.K. Y.I. Y.K. J.T. and K.N. have no competing interests. Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = apr,
day = "14",
doi = "10.1016/j.stemcr.2020.02.008",
language = "English",
volume = "14",
pages = "663--676",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "4",
}