Transplantation of hiPSC-derived corneal epithelial cell (iCEC) sheets is expected to serve as a treatment for severe corneal diseases. The efficacy of this treatment depends on effective iCEC purification. Hayashi and colleagues used differential laminin adhesion of iCECs and non-CECs in combination magnetic cell sorting to develop a simple method to obtain highly pure iCEC sheets without using FACS.
- cell competition
- cell purification
- corneal epithelial cells (CECs)
- human induced pluripotent stem cells (hiPSCs)
- laminin isoforms
- substrate-specific adhesion