Cellular localization of IL-18 and IL-18 receptor in pig anterior pituitary gland

Yasuhiro Nagai, Kouichi Watanabe, Hisashi Aso, Shyuichi Ohwada, Yoshihiro Muneta, Takahiro Yamaguchi

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    14 Citations (Scopus)


    Pro-inflammatory cytokine interleukin 18 (IL-18) has been proposed to have a role in modulating immuno-endocrine functions. Our previous study showed that IL-18 and IL-18 receptor (IL-18R) colocalized in somatotrophs of the bovine anterior pituitary gland, and the possibility that IL-18 acts on somatotrophs as an autocrine factor. In the present study, we investigated the localization of IL-18 and IL-18R in the pig anterior pituitary gland. RT-PCR analysis showed the expression of IL-18 and IL-18R mRNAin the pig anterior pituitary gland. Immunohistochemistry of IL-18 and specific hormones revealed the presence of IL-18 in somatotrophs, mammotrophs, thyrotrophs and gonadotrophs. IL-18R was localized in somatotrophs and thyrotrophs. Furthermore, the somatotrophs immunoreactive for IL-18 did not contain IL-18R. Thus, IL-18R and IL-18 were not colocalized in an identical somatotroph. These findings suggest that the localization of IL-18 in pig somatotrophs is different from that in bovine somatotrophs, although IL-18 closely associates with somatotrophs in the anterior pituitary glands in both species.

    Original languageEnglish
    Pages (from-to)144-154
    Number of pages11
    JournalDomestic Animal Endocrinology
    Issue number2
    Publication statusPublished - 2006 Feb


    • Immuno-endocrine signalling
    • Interleukin-18
    • Interleukin-18R
    • Paracrine
    • Pig anterior pituitary gland

    ASJC Scopus subject areas

    • Food Animals
    • Animal Science and Zoology
    • Endocrinology


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