Change in infected root canal microflora during the course of root canal therapy

Keiko Yamaki; Takuichi Sato; Ayako Hasegawa; Yuki Abiko; Kazuhiro Hashimoto; Yasuhisa Takeuchi; Junko Matsuyama; Hidetoshi Shimauchi; Nobuhiro Takahashi

doi:10.1007/978-4-431-54070-0_62

Change in infected root canal microflora during the course of root canal therapy

Keiko Yamaki, Takuichi Sato, Ayako Hasegawa, Yuki Abiko, Kazuhiro Hashimoto, Yasuhisa Takeuchi, Junko Matsuyama, Hidetoshi Shimauchi, Nobuhiro Takahashi

DEN - Dental Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The purpose of the present study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacte- rial identification, before and after root canal treatment. The mean bacterial count in root canals before treatment was 106, and obligate anaerobes were predominant. The predominant isolates were Olsenella, Pseudoramibacter, Propionibacterium and Mogibacterium. On second and third visits, no bacteria were detected in the samples collected after treatment. These results suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.

Original language	English
Title of host publication	Interface Oral Health Science 2011
Publisher	Springer Japan
Pages	181-182
Number of pages	2
ISBN (Electronic)	9784431540700
ISBN (Print)	9784431540694
DOIs	https://doi.org/10.1007/978-4-431-54070-0_62
Publication status	Published - 2012 Jan 1

Keywords

16S rRNA
Infected root canal
Microbiota
PCR
Root canal therapy

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10.1007/978-4-431-54070-0_62

Cite this

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abstract = "The purpose of the present study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacte- rial identification, before and after root canal treatment. The mean bacterial count in root canals before treatment was 106, and obligate anaerobes were predominant. The predominant isolates were Olsenella, Pseudoramibacter, Propionibacterium and Mogibacterium. On second and third visits, no bacteria were detected in the samples collected after treatment. These results suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.",

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AU - Yamaki, Keiko

AU - Sato, Takuichi

AU - Hasegawa, Ayako

AU - Abiko, Yuki

AU - Hashimoto, Kazuhiro

AU - Takeuchi, Yasuhisa

AU - Matsuyama, Junko

AU - Shimauchi, Hidetoshi

AU - Takahashi, Nobuhiro

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N2 - The purpose of the present study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacte- rial identification, before and after root canal treatment. The mean bacterial count in root canals before treatment was 106, and obligate anaerobes were predominant. The predominant isolates were Olsenella, Pseudoramibacter, Propionibacterium and Mogibacterium. On second and third visits, no bacteria were detected in the samples collected after treatment. These results suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.

AB - The purpose of the present study was to profile the microflora in infected root canals, using anaerobic culture and molecular biological techniques for bacte- rial identification, before and after root canal treatment. The mean bacterial count in root canals before treatment was 106, and obligate anaerobes were predominant. The predominant isolates were Olsenella, Pseudoramibacter, Propionibacterium and Mogibacterium. On second and third visits, no bacteria were detected in the samples collected after treatment. These results suggest that the environment in root canals is anaerobic and therefore support the growth of anaerobes, and that adequate treatment changes the root canal environment drastically.

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KW - Microbiota

KW - PCR

KW - Root canal therapy

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Change in infected root canal microflora during the course of root canal therapy

Abstract

Keywords

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