Changes in the liver transcriptome and physiological parameters of Japanese Black steers during the fattening period

Minji Kim, Tatsunori Masaki, Kentaro Ikuta, Eiji Iwamoto, Yoshinobu Uemoto, Fuminori Terada, Sanggun Roh

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


We investigated the physiological changes during the fattening period and production characteristics in Japanese Black steers bred and raised using the typical feeding system in Japan. Here, 21 Japanese Black steers aged 12 months were used, with experimental period divided into early (12–14 months of age), middle (15–22 months), and late fattening phases (23–30 months). The liver transcriptome, blood metabolites, hormones, and rumen fermentation characteristics were analyzed. Blood triglyceride and non-esterified fatty acid concentrations increased, whereas blood ketone levels decreased, with fattening phases. Blood insulin increased with fattening phases and was positively correlated with carcass weight and marbling in late fattening phases. Rumen fermentation characteristics showed high propionate levels and low butyrate levels in late fattening phases, likely due to increased energy intake. Genes related to glucose metabolism, such as SESN3, INSR, LEPR, and FOXO3, were down-regulated in late fattening phases. Genes related to lipid metabolism, such as FABP4, were up-regulated, whereas FADS1 and FADS2 were down-regulated. These findings suggest that the physiological changes resulted from changes in the energy content and composition of diets. Liver metabolism changed with changes in fat metabolism. Insulin was strongly associated with physiological changes and productivity in Japanese Black cattle.

Original languageEnglish
Article number4029
JournalScientific reports
Issue number1
Publication statusPublished - 2022 Dec
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Changes in the liver transcriptome and physiological parameters of Japanese Black steers during the fattening period'. Together they form a unique fingerprint.

Cite this