TY - JOUR
T1 - Changes in the morphology of cell-size liposomes in the presence of cholesterol
T2 - Formation of neuron-like tubes and liposome networks
AU - Nomura, Shin Ichiro M.
AU - Mizutani, Yumi
AU - Kurita, Kimio
AU - Watanabe, Akihiko
AU - Akiyoshi, Kazunari
N1 - Funding Information:
The LSM device was kindly provided by Prof. I. Morita at Tokyo Medical and Dental Univ. This research was supported by a grant from the Center of Excellence Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone at Tokyo Medical and Dental University. This work was also supported by a Grant-in-Aid for Scientific Research from the Japanese Government (No. 15650090).
PY - 2005/5/20
Y1 - 2005/5/20
N2 - Spontaneous changes in the morphology of cell-size liposomes (dioleoylphosphatidylcholine, DOPC and egg PC) as model cells were investigated in the presence of cholesterol. Tube structures and liposome networks connected by the tubes were observed in the presence of 5-30% cholesterol by dark-field and laser-scanning microscopy. Furthermore, in the presence of more than 40 mol% of cholesterol, the tubes disappeared and changed to small liposomes. Thus, cholesterol induced a morphological change in giant liposomes from tubes to small liposomes. These phenomena may be related to the role of cholesterol in the morphological changes in living cells such as neurons.
AB - Spontaneous changes in the morphology of cell-size liposomes (dioleoylphosphatidylcholine, DOPC and egg PC) as model cells were investigated in the presence of cholesterol. Tube structures and liposome networks connected by the tubes were observed in the presence of 5-30% cholesterol by dark-field and laser-scanning microscopy. Furthermore, in the presence of more than 40 mol% of cholesterol, the tubes disappeared and changed to small liposomes. Thus, cholesterol induced a morphological change in giant liposomes from tubes to small liposomes. These phenomena may be related to the role of cholesterol in the morphological changes in living cells such as neurons.
KW - Cholesterol
KW - Giant liposome
KW - Lipid bilayer
KW - Neuron
KW - Tubular network
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U2 - 10.1016/j.bbamem.2005.02.005
DO - 10.1016/j.bbamem.2005.02.005
M3 - Article
C2 - 15893519
AN - SCOPUS:18944407057
SN - 0005-2736
VL - 1669
SP - 164
EP - 169
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 2
ER -