TY - JOUR
T1 - Characterisation of sugar nucleotides in colostrum of dairy domestic farms animals
AU - Mineguchi, Yuri
AU - Goto, Kyosuke
AU - Sudo, Yuna
AU - Hirayama, Kentaro
AU - Kashiwagi, Hirotoshi
AU - Sasagase, Izumi
AU - Kitazawa, Haruki
AU - Asakuma, Sadaki
AU - Fukuda, Kenji
AU - Urashima, Tadasu
N1 - Funding Information:
We thank Mr. Kazu Nakai, Mr. Nozomi Miyajima, Mr. Michihiko Koshiyama, Mr. Hiroshi Mutoh and Mr. Yoshiki Yagyu for supplying colostrum samples. This work was supported by a Grant-in-Aid for Scientific Research ©, grant No. 19K06364 , from Japan Society of the Promotion of Science (JSPS) , and a grant from Yotsuba Milk Products Co. (Japan).
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2021/2
Y1 - 2021/2
N2 - The biological significance and heterogeneity of bovine, caprine and ovine colostrum sugar nucleotides is unclear. Colostrum sugar nucleotides and sugar monophosphates were purified and characterised with 1H-NMR spectroscopy. UDP-galactose (UDP-Gal) and UDP-glucose (UDP-Glc) were identified in colostrum of Holstein and Brown Swiss cows; UDP-Gal, UDP-Glc, UDP-N-acetylgalactosamine (UDP-GalNAc) and UDP-N-acetylglucosamine (UDP-GlcNAc) were found in Japanese Saanen goat colostrum. UDP-Gal, UDP-Glc, UDP-GalNAc and UDP-GlcNAc were found in Corriedale sheep but not in the Suffolk and Fraise Land breeds. The presence/absence of these sugar nucleotides in colostrum is heterogeneous among dairy domestic farm animals and the three sheep breeds. In addition, a nucleotide, uridine-ribose diphosphate (UDP) was identified in the colostra of Japanese Saanen goat, and Suffolk and Fraiseland sheep, while uridine-ribose monophosphate (UMP) was detected in the colostrum of Brown Swiss cow and the three breed sheep. N-acetylglucosamine-1-phosphate (GlcNAc-1-P) was also identified in the colostrum of the goat and two cow breeds.
AB - The biological significance and heterogeneity of bovine, caprine and ovine colostrum sugar nucleotides is unclear. Colostrum sugar nucleotides and sugar monophosphates were purified and characterised with 1H-NMR spectroscopy. UDP-galactose (UDP-Gal) and UDP-glucose (UDP-Glc) were identified in colostrum of Holstein and Brown Swiss cows; UDP-Gal, UDP-Glc, UDP-N-acetylgalactosamine (UDP-GalNAc) and UDP-N-acetylglucosamine (UDP-GlcNAc) were found in Japanese Saanen goat colostrum. UDP-Gal, UDP-Glc, UDP-GalNAc and UDP-GlcNAc were found in Corriedale sheep but not in the Suffolk and Fraise Land breeds. The presence/absence of these sugar nucleotides in colostrum is heterogeneous among dairy domestic farm animals and the three sheep breeds. In addition, a nucleotide, uridine-ribose diphosphate (UDP) was identified in the colostra of Japanese Saanen goat, and Suffolk and Fraiseland sheep, while uridine-ribose monophosphate (UMP) was detected in the colostrum of Brown Swiss cow and the three breed sheep. N-acetylglucosamine-1-phosphate (GlcNAc-1-P) was also identified in the colostrum of the goat and two cow breeds.
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U2 - 10.1016/j.idairyj.2020.104897
DO - 10.1016/j.idairyj.2020.104897
M3 - Article
AN - SCOPUS:85095440614
SN - 0958-6946
VL - 113
JO - International Dairy Journal
JF - International Dairy Journal
M1 - 104897
ER -