Characteristic effects of methylglyoxal and its degraded product formate on viability of human histiocytes: A possible detoxification pathway of methylglyoxal

Kenji Koizumi; Masaaki Nakayama; Wan Jun Zhu; Sadayoshi Ito

doi:10.1016/j.bbrc.2011.03.041

Characteristic effects of methylglyoxal and its degraded product formate on viability of human histiocytes: A possible detoxification pathway of methylglyoxal

Kenji Koizumi, Masaaki Nakayama, Wan Jun Zhu, Sadayoshi Ito

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Methylglyoxal (MGO) is a toxic and highly reactive alpha-oxoaldehyde, elevated in the states of various diseases underlying enhanced oxidative stress. Furthermore, MGO has been reported to generate another aldehyde, formic acid (FA). In this sense, investigating the biological property of FA is crucially important. The present study examined the effects of MGO and FA on cell viability using the U937 human histiocytic cell line. FA showed a dose-dependent increase in cell viability at the concentrations of MGO in which cell viability decreased. The mechanism of the increase by FA involved the presence of endogenous hydrogen peroxide (H₂O₂) and tetrahydrofolate in the folate pathway, whereas that of the decrease in cell viability by MGO involved interaction with H₂O₂ and oxidative damage. These findings suggest that FA production by MGO degradation may play a role in attenuation of oxidative cellular injury caused by MGO. We hypothesize that FA generation pathway constitutes a detoxification system for MGO.

Original language	English
Pages (from-to)	426-431
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	407
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2011.03.041
Publication status	Published - 2011 Apr 8

Keywords

Formic acid
Methylglyoxal
Oxidative stress
Purine metabolism
Viability

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2011.03.041

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Characteristic effects of methylglyoxal and its degraded product formate on viability of human histiocytes: A possible detoxification pathway of methylglyoxal. / Koizumi, Kenji; Nakayama, Masaaki; Zhu, Wan Jun et al.
In: Biochemical and biophysical research communications, Vol. 407, No. 2, 08.04.2011, p. 426-431.

Research output: Contribution to journal › Article › peer-review

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abstract = "Methylglyoxal (MGO) is a toxic and highly reactive alpha-oxoaldehyde, elevated in the states of various diseases underlying enhanced oxidative stress. Furthermore, MGO has been reported to generate another aldehyde, formic acid (FA). In this sense, investigating the biological property of FA is crucially important. The present study examined the effects of MGO and FA on cell viability using the U937 human histiocytic cell line. FA showed a dose-dependent increase in cell viability at the concentrations of MGO in which cell viability decreased. The mechanism of the increase by FA involved the presence of endogenous hydrogen peroxide (H2O2) and tetrahydrofolate in the folate pathway, whereas that of the decrease in cell viability by MGO involved interaction with H2O2 and oxidative damage. These findings suggest that FA production by MGO degradation may play a role in attenuation of oxidative cellular injury caused by MGO. We hypothesize that FA generation pathway constitutes a detoxification system for MGO.",

keywords = "Formic acid, Methylglyoxal, Oxidative stress, Purine metabolism, Viability",

author = "Kenji Koizumi and Masaaki Nakayama and Zhu, {Wan Jun} and Sadayoshi Ito",

note = "Funding Information: This work is supported by Grants partly from Astellas Pharma Inc. to M.N. The authors wish to express their special thanks to Prof. M. Fukumoto, at Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University , for his appropriate advice, and Ms. A. Momoi, Ms. M. Nakayama, and Ms. J. Sakaino, for their excellent assistance for the study. ",

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AU - Zhu, Wan Jun

AU - Ito, Sadayoshi

N1 - Funding Information: This work is supported by Grants partly from Astellas Pharma Inc. to M.N. The authors wish to express their special thanks to Prof. M. Fukumoto, at Department of Pathology, Institute of Development, Aging and Cancer, Tohoku University , for his appropriate advice, and Ms. A. Momoi, Ms. M. Nakayama, and Ms. J. Sakaino, for their excellent assistance for the study.

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N2 - Methylglyoxal (MGO) is a toxic and highly reactive alpha-oxoaldehyde, elevated in the states of various diseases underlying enhanced oxidative stress. Furthermore, MGO has been reported to generate another aldehyde, formic acid (FA). In this sense, investigating the biological property of FA is crucially important. The present study examined the effects of MGO and FA on cell viability using the U937 human histiocytic cell line. FA showed a dose-dependent increase in cell viability at the concentrations of MGO in which cell viability decreased. The mechanism of the increase by FA involved the presence of endogenous hydrogen peroxide (H2O2) and tetrahydrofolate in the folate pathway, whereas that of the decrease in cell viability by MGO involved interaction with H2O2 and oxidative damage. These findings suggest that FA production by MGO degradation may play a role in attenuation of oxidative cellular injury caused by MGO. We hypothesize that FA generation pathway constitutes a detoxification system for MGO.

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Characteristic effects of methylglyoxal and its degraded product formate on viability of human histiocytes: A possible detoxification pathway of methylglyoxal

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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