TY - JOUR
T1 - Characteristic Profiles of Inflammatory Cytokines in the Aqueous Humor of Glaucomatous Eyes
AU - Kokubun, Taiki
AU - Tsuda, Satoru
AU - Kunikata, Hiroshi
AU - Yasuda, Masayuki
AU - Himori, Noriko
AU - Kunimatsu-Sanuki, Shiho
AU - Maruyama, Kazuichi
AU - Nakazawa, Toru
N1 - Funding Information:
This paper was supported in part by a JST grant from JSPS KAKENHI Grants-in-Aid for Scientific Research (B) (T.N. 26293372). The funder had no role in design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; or the decision to submit the manuscript for publication.
Publisher Copyright:
©, © Taylor & Francis Group, LLC.
PY - 2018/11/17
Y1 - 2018/11/17
N2 - Purpose: To analyze cytokine profiles of the aqueous humor of eyes with primary open-angle glaucoma (POAG), neovascular glaucoma (NVG), and cataract (as controls). Methods: A multiplex bead assay was used to measure concentrations of 27 cytokines in aqueous humor samples from 54 eyes. Results: Detection rates were as follows: IL-7: NVG higher than POAG; IL-10: POAG lower than cataract or NVG; and GM-CSF: cataract higher than POAG or NVG. Concentrations were as follows: IL-8, IP-10, MCP-1, and MIP-1β: POAG and NVG higher than cataract; IL-9: POAG lower than NVG; IL-12: POAG lower than cataract or NVG; and VEGF: NVG higher than cataract or POAG and POAG lower than cataract. Further analysis showed that IL-8, IP-10, MCP-1, and MIP-1β were correlated with intraocular pressure and age. Conclusions: The detection rates and levels of various cytokines had different patterns in POAG and NVG patients, suggesting distinctive alterations in the microenvironment in different types of glaucoma.
AB - Purpose: To analyze cytokine profiles of the aqueous humor of eyes with primary open-angle glaucoma (POAG), neovascular glaucoma (NVG), and cataract (as controls). Methods: A multiplex bead assay was used to measure concentrations of 27 cytokines in aqueous humor samples from 54 eyes. Results: Detection rates were as follows: IL-7: NVG higher than POAG; IL-10: POAG lower than cataract or NVG; and GM-CSF: cataract higher than POAG or NVG. Concentrations were as follows: IL-8, IP-10, MCP-1, and MIP-1β: POAG and NVG higher than cataract; IL-9: POAG lower than NVG; IL-12: POAG lower than cataract or NVG; and VEGF: NVG higher than cataract or POAG and POAG lower than cataract. Further analysis showed that IL-8, IP-10, MCP-1, and MIP-1β were correlated with intraocular pressure and age. Conclusions: The detection rates and levels of various cytokines had different patterns in POAG and NVG patients, suggesting distinctive alterations in the microenvironment in different types of glaucoma.
KW - Aqueous humor
KW - cytokine
KW - glaucoma
KW - NVG
KW - POAG
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U2 - 10.1080/09273948.2017.1327605
DO - 10.1080/09273948.2017.1327605
M3 - Article
C2 - 28622063
AN - SCOPUS:85020541814
SN - 0927-3948
VL - 26
SP - 1177
EP - 1188
JO - Ocular Immunology and Inflammation
JF - Ocular Immunology and Inflammation
IS - 8
ER -