TY - JOUR
T1 - Characteristics of alpha smooth muscle actin-positive peritubular cells in prepubertal bovine testes
AU - Kawabe, Yusuke
AU - Numabe, Takashi
AU - Tanemura, Kentaro
AU - Hara, Kenshiro
N1 - Funding Information:
We thank to A. Suzuki, S. Yaguchi, E. Kageyama, R. Yanai, Y. Hiradate and S. Takahashi for technical assistance, and S. Abe for secretarial assistance. This work was supported by Grant-in-Aid for Scientific Research ( KAKENHI ; 19H05237 , 19K22358 , 21H02341 , 21K19175 ), AMED-CREST ( 21gm1110005s0205 ), JST FOREST Program ( JPMJFR2018 ) and the Sumitomo-Foundation ( 190421 ) to K.H. and Suisei Nougaku Shinkoukai-Foundation ( J210002677 ) to Y. K.
Publisher Copyright:
© 2022 The Authors
PY - 2022/6/18
Y1 - 2022/6/18
N2 - Characteristics of peritubular myoid cells (PMCs) in sexually immature cattle remain largely unknown. Here, we report the character and behavior of peritubular cells expressing alpha smooth muscle actin (αSMA), a marker of PMCs in adult testes, in prepubertal testes procured from 5-months-old bulls. Elastin distribution around αSMA + PMCs was dim and discontinuous in prepubertal testes, but strong and continuous in adult testes. Fibroblast-specific protein 1 (FSP1) was rarely expressed in αSMA + PMCs of prepubertal testes, while in adult testes, majority of αSMA + PMCs were FSP1+. Moreover, αSMA + PMCs in prepubertal testes proliferated more actively than those in adult testes. In vitro culture of isolated seminiferous tubules from prepubertal testes revealed that αSMA + PMCs migrate from peritubular to interstitial area. Hence, in prepubertal bovine testes, (1) PMCs rarely portray fibroblast-like properties, (2) PMCs exhibit heterogeneity in FSP1 expression, (3) PMCs proliferate more actively than those in adult testes, and (4) PMCs have a potential to migrate to the interstitium. Our observations help to understand the maturation of PMCs and their involvement in bovine testicular function.
AB - Characteristics of peritubular myoid cells (PMCs) in sexually immature cattle remain largely unknown. Here, we report the character and behavior of peritubular cells expressing alpha smooth muscle actin (αSMA), a marker of PMCs in adult testes, in prepubertal testes procured from 5-months-old bulls. Elastin distribution around αSMA + PMCs was dim and discontinuous in prepubertal testes, but strong and continuous in adult testes. Fibroblast-specific protein 1 (FSP1) was rarely expressed in αSMA + PMCs of prepubertal testes, while in adult testes, majority of αSMA + PMCs were FSP1+. Moreover, αSMA + PMCs in prepubertal testes proliferated more actively than those in adult testes. In vitro culture of isolated seminiferous tubules from prepubertal testes revealed that αSMA + PMCs migrate from peritubular to interstitial area. Hence, in prepubertal bovine testes, (1) PMCs rarely portray fibroblast-like properties, (2) PMCs exhibit heterogeneity in FSP1 expression, (3) PMCs proliferate more actively than those in adult testes, and (4) PMCs have a potential to migrate to the interstitium. Our observations help to understand the maturation of PMCs and their involvement in bovine testicular function.
KW - Alpha smooth muscle actin
KW - Fibroblast specific protein 1
KW - Organ culture
KW - Peritubular myoid cells
KW - Prepubertal bovine testes
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U2 - 10.1016/j.bbrc.2022.03.149
DO - 10.1016/j.bbrc.2022.03.149
M3 - Article
C2 - 35413539
AN - SCOPUS:85127766494
SN - 0006-291X
VL - 609
SP - 48
EP - 53
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
ER -