Characterization and potential of three temperature ranges for hydrogen fermentation of cellulose by means of activity test and 16s rRNA sequence analysis

Samir I. Gadow; Hongyu Jiang; Yu You Li

doi:10.1016/j.biortech.2016.02.098

Characterization and potential of three temperature ranges for hydrogen fermentation of cellulose by means of activity test and 16s rRNA sequence analysis

Samir I. Gadow, Hongyu Jiang, Yu You Li

ENG - Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A series of standardized activity experiments were performed to characterize three different temperature ranges of hydrogen fermentation from different carbon sources. 16S rRNA sequences analysis showed that the bacteria were close to Enterobacter genus in the mesophilic mixed culture (MMC) and Thermoanaerobacterium genus in the thermophilic and hyper-thermophilic mixed cultures (TMC and HMC). The MMC was able to utilize the glucose and cellulose to produce methane gas within a temperature range between 25 and 45 °C and hydrogen gas from 35 to 60 °C. While, the TMC and HMC produced only hydrogen gas at all temperature ranges and the highest activity of 521.4 ml H₂/g VSS d was obtained by TMC. The thermodynamic analysis showed that more energy is consumed by hydrogen production from cellulose than from glucose. The experimental results could help to improve the economic feasibility of cellulosic biomass energy using three-phase technology to produce hythane.

Original language	English
Pages (from-to)	80-89
Number of pages	10
Journal	Bioresource Technology
Volume	209
DOIs	https://doi.org/10.1016/j.biortech.2016.02.098
Publication status	Published - 2016 Jan 1

Keywords

16s rRNA
Bio-hydrogen
Cellulose
Mixed culture
Temperature variation

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2016.02.098

Cite this

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title = "Characterization and potential of three temperature ranges for hydrogen fermentation of cellulose by means of activity test and 16s rRNA sequence analysis",

abstract = "A series of standardized activity experiments were performed to characterize three different temperature ranges of hydrogen fermentation from different carbon sources. 16S rRNA sequences analysis showed that the bacteria were close to Enterobacter genus in the mesophilic mixed culture (MMC) and Thermoanaerobacterium genus in the thermophilic and hyper-thermophilic mixed cultures (TMC and HMC). The MMC was able to utilize the glucose and cellulose to produce methane gas within a temperature range between 25 and 45 °C and hydrogen gas from 35 to 60 °C. While, the TMC and HMC produced only hydrogen gas at all temperature ranges and the highest activity of 521.4 ml H2/g VSS d was obtained by TMC. The thermodynamic analysis showed that more energy is consumed by hydrogen production from cellulose than from glucose. The experimental results could help to improve the economic feasibility of cellulosic biomass energy using three-phase technology to produce hythane.",

keywords = "16s rRNA, Bio-hydrogen, Cellulose, Mixed culture, Temperature variation",

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T1 - Characterization and potential of three temperature ranges for hydrogen fermentation of cellulose by means of activity test and 16s rRNA sequence analysis

AU - Gadow, Samir I.

AU - Jiang, Hongyu

AU - Li, Yu You

PY - 2016/1/1

Y1 - 2016/1/1

N2 - A series of standardized activity experiments were performed to characterize three different temperature ranges of hydrogen fermentation from different carbon sources. 16S rRNA sequences analysis showed that the bacteria were close to Enterobacter genus in the mesophilic mixed culture (MMC) and Thermoanaerobacterium genus in the thermophilic and hyper-thermophilic mixed cultures (TMC and HMC). The MMC was able to utilize the glucose and cellulose to produce methane gas within a temperature range between 25 and 45 °C and hydrogen gas from 35 to 60 °C. While, the TMC and HMC produced only hydrogen gas at all temperature ranges and the highest activity of 521.4 ml H2/g VSS d was obtained by TMC. The thermodynamic analysis showed that more energy is consumed by hydrogen production from cellulose than from glucose. The experimental results could help to improve the economic feasibility of cellulosic biomass energy using three-phase technology to produce hythane.

AB - A series of standardized activity experiments were performed to characterize three different temperature ranges of hydrogen fermentation from different carbon sources. 16S rRNA sequences analysis showed that the bacteria were close to Enterobacter genus in the mesophilic mixed culture (MMC) and Thermoanaerobacterium genus in the thermophilic and hyper-thermophilic mixed cultures (TMC and HMC). The MMC was able to utilize the glucose and cellulose to produce methane gas within a temperature range between 25 and 45 °C and hydrogen gas from 35 to 60 °C. While, the TMC and HMC produced only hydrogen gas at all temperature ranges and the highest activity of 521.4 ml H2/g VSS d was obtained by TMC. The thermodynamic analysis showed that more energy is consumed by hydrogen production from cellulose than from glucose. The experimental results could help to improve the economic feasibility of cellulosic biomass energy using three-phase technology to produce hythane.

KW - 16s rRNA

KW - Bio-hydrogen

KW - Cellulose

KW - Mixed culture

KW - Temperature variation

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JF - Agricultural Wastes

ER -

Characterization and potential of three temperature ranges for hydrogen fermentation of cellulose by means of activity test and 16s rRNA sequence analysis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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