Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27

Junichi Miyazaki, Nobuyuki Kobashi, Tomoyuki Fujii, Makoto Nishiyama, Hisakazu Yamane

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


We conducted a chromosome walk to obtain a DNA fragment downstream of lysJ and found an argE homolog in a putative operon composed of lysJ-orfC-orfD-argE homologs. A knockout mutant of the argE homolog showed significantly slow growth on a minimal medium, and the growth was markedly improved by addition of lysine. We therefore termed this gene lysK. Purified LysK protein has deacetylating activities for both N2-acetyllysine and N2-acetylornithine at almost equal efficiency. These results suggest that lysK which may share an ancestor with argE functions not only for the lysine biosynthesis, but also for arginine biosynthesis in Thermus thermophilus.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalFEBS Letters
Issue number1-3
Publication statusPublished - 2002 Feb 13


  • Arginine biosynthesis
  • Lysine biosynthesis
  • Thermus thermophilus
  • argE


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