Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27

Junichi Miyazaki; Nobuyuki Kobashi; Tomoyuki Fujii; Makoto Nishiyama; Hisakazu Yamane

doi:10.1016/S0014-5793(02)02290-1

Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27

Junichi Miyazaki, Nobuyuki Kobashi, Tomoyuki Fujii, Makoto Nishiyama, Hisakazu Yamane

AGR - Agricultural Chemistry

The University of Tokyo

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We conducted a chromosome walk to obtain a DNA fragment downstream of lysJ and found an argE homolog in a putative operon composed of lysJ-orfC-orfD-argE homologs. A knockout mutant of the argE homolog showed significantly slow growth on a minimal medium, and the growth was markedly improved by addition of lysine. We therefore termed this gene lysK. Purified LysK protein has deacetylating activities for both N²-acetyllysine and N²-acetylornithine at almost equal efficiency. These results suggest that lysK which may share an ancestor with argE functions not only for the lysine biosynthesis, but also for arginine biosynthesis in Thermus thermophilus.

Original language	English
Pages (from-to)	269-274
Number of pages	6
Journal	FEBS Letters
Volume	512
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(02)02290-1
Publication status	Published - 2002 Feb 13

Keywords

Arginine biosynthesis
Lysine biosynthesis
Thermus thermophilus
argE

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10.1016/S0014-5793(02)02290-1

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title = "Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27",

abstract = "We conducted a chromosome walk to obtain a DNA fragment downstream of lysJ and found an argE homolog in a putative operon composed of lysJ-orfC-orfD-argE homologs. A knockout mutant of the argE homolog showed significantly slow growth on a minimal medium, and the growth was markedly improved by addition of lysine. We therefore termed this gene lysK. Purified LysK protein has deacetylating activities for both N2-acetyllysine and N2-acetylornithine at almost equal efficiency. These results suggest that lysK which may share an ancestor with argE functions not only for the lysine biosynthesis, but also for arginine biosynthesis in Thermus thermophilus.",

keywords = "Arginine biosynthesis, Lysine biosynthesis, Thermus thermophilus, argE",

author = "Junichi Miyazaki and Nobuyuki Kobashi and Tomoyuki Fujii and Makoto Nishiyama and Hisakazu Yamane",

note = "Funding Information: We thank Dr. H. Nishida (Institute of Molecular and Cellular Biosciences, The University of Tokyo) for his assistance in phylogenetic analysis. This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.",

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doi = "10.1016/S0014-5793(02)02290-1",

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T1 - Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27

AU - Miyazaki, Junichi

AU - Kobashi, Nobuyuki

AU - Fujii, Tomoyuki

AU - Nishiyama, Makoto

AU - Yamane, Hisakazu

N1 - Funding Information: We thank Dr. H. Nishida (Institute of Molecular and Cellular Biosciences, The University of Tokyo) for his assistance in phylogenetic analysis. This work was supported in part by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

PY - 2002/2/13

Y1 - 2002/2/13

N2 - We conducted a chromosome walk to obtain a DNA fragment downstream of lysJ and found an argE homolog in a putative operon composed of lysJ-orfC-orfD-argE homologs. A knockout mutant of the argE homolog showed significantly slow growth on a minimal medium, and the growth was markedly improved by addition of lysine. We therefore termed this gene lysK. Purified LysK protein has deacetylating activities for both N2-acetyllysine and N2-acetylornithine at almost equal efficiency. These results suggest that lysK which may share an ancestor with argE functions not only for the lysine biosynthesis, but also for arginine biosynthesis in Thermus thermophilus.

AB - We conducted a chromosome walk to obtain a DNA fragment downstream of lysJ and found an argE homolog in a putative operon composed of lysJ-orfC-orfD-argE homologs. A knockout mutant of the argE homolog showed significantly slow growth on a minimal medium, and the growth was markedly improved by addition of lysine. We therefore termed this gene lysK. Purified LysK protein has deacetylating activities for both N2-acetyllysine and N2-acetylornithine at almost equal efficiency. These results suggest that lysK which may share an ancestor with argE functions not only for the lysine biosynthesis, but also for arginine biosynthesis in Thermus thermophilus.

KW - Arginine biosynthesis

KW - Lysine biosynthesis

KW - Thermus thermophilus

KW - argE

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DO - 10.1016/S0014-5793(02)02290-1

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AN - SCOPUS:0037070156

SN - 0014-5793

VL - 512

SP - 269

EP - 274

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27

Abstract

Keywords

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