Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10

Shun Ichiro Maruyama, Shigetsugu Hatakeyama, Keiko Nakayama, Noriko Ishida, Koichi Kawakami, Kei Ichi Nakayama

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


The SCF complex is a type of ubiquitin ligase that consists of the invariable components SKP1, CUL1, and RBX1 as well as a variable component, known as an F-box protein, that is the main determinant of substrate specificity. The Caenorhabditis elegans F-box- and WD40-repeat-containing protein SEL-10 functionally and physically associates with LIN-12 and SEL-12, orthologues of mammalian Notch and presenilin, respectively. We have now identified a gene (which we call Fbxw6) that encodes a mouse homologue (F-box-WD40 repeat protein 6, or FBW6) of SEL-10 and is expressed mainly in brain, heart, and testis. Co-immuno-precipitation analysis showed that FBW6 interacts with SKP1 and CUL1, indicating that these three proteins form an SCF complex. Comparison of the genomic organization of Fbxw6, which is located on mouse chromosome 3.3E3, with that of mouse Fbxw1, Fbxw2, and Fbxw4 showed only a low level of similarity, indicating that these genes diverged relatively early and thereafter evolved independently.

Original languageEnglish
Pages (from-to)214-222
Number of pages9
Issue number3
Publication statusPublished - 2001 Sept 15


  • F-box protein
  • FBW6
  • Notch
  • Presenilin
  • Proteolysis
  • SCF complex
  • SEL-10
  • Ubiquitin
  • Ubiquitin ligase
  • WD40 repeat


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