Characterization of alpha-amylase from aspergillus niger aggregate F isolated from a fermented cassava gatot grown in potato peel waste medium

Cindy Angelia; Astia Sanjaya; Aida; Ellen Tanudjaja; Hans Victor; Antari Daru Cahyani; Tjie Jan Tan; Reinhard Pinontoan

doi:10.4014/mbl.1811.11011

Characterization of alpha-amylase from aspergillus niger aggregate F isolated from a fermented cassava gatot grown in potato peel waste medium

Cindy Angelia, Astia Sanjaya, Aida, Ellen Tanudjaja, Hans Victor, Antari Daru Cahyani, Tjie Jan Tan, Reinhard Pinontoan

ENG - Biomolecular Engineering

Pelita Harapan University

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The use of GRAS microorganisms isolated from fermented foods during amylase production using an economical food-waste medium provides more opportunities to produce amylase with a wider range of applications. Hence, this study aimed to isolate a good amylase-producing fungi from the traditional Indonesian fermented cassava, gatot, and to identify the amylase-producing capability of the isolate in a potato peel waste (PPW) medium. Black-colored fungi isolated from gatot was morphologically identified and the amylase produced was characterized using SDS-PAGE and Native PAGE. The isolate was then grown on PPW medium, and the amylase produced was further characterized. Morphological identification and enzyme characterization revealed that the Aspergillus niger aggregate F isolated from gatot secreted an active extracellular α-amylase with an optimum pH of 5-6. In conclusion, Aspergillus niger aggregate F isolated from gatot can be used to produce α-amylase using PPW as a medium.

Original language	English
Pages (from-to)	364-371
Number of pages	8
Journal	Microbiology and Biotechnology Letters
Volume	47
Issue number	3
DOIs	https://doi.org/10.4014/mbl.1811.11011
Publication status	Published - 2019 Sept 28

Keywords

Alpha-amylase
Aspergillus Niger
Fermented cassava gatot
Potato peel waste

Access to Document

10.4014/mbl.1811.11011

Cite this

@article{3a727f2c705541aca9ab7339c273052f,

title = "Characterization of alpha-amylase from aspergillus niger aggregate F isolated from a fermented cassava gatot grown in potato peel waste medium",

abstract = "The use of GRAS microorganisms isolated from fermented foods during amylase production using an economical food-waste medium provides more opportunities to produce amylase with a wider range of applications. Hence, this study aimed to isolate a good amylase-producing fungi from the traditional Indonesian fermented cassava, gatot, and to identify the amylase-producing capability of the isolate in a potato peel waste (PPW) medium. Black-colored fungi isolated from gatot was morphologically identified and the amylase produced was characterized using SDS-PAGE and Native PAGE. The isolate was then grown on PPW medium, and the amylase produced was further characterized. Morphological identification and enzyme characterization revealed that the Aspergillus niger aggregate F isolated from gatot secreted an active extracellular α-amylase with an optimum pH of 5-6. In conclusion, Aspergillus niger aggregate F isolated from gatot can be used to produce α-amylase using PPW as a medium.",

keywords = "Alpha-amylase, Aspergillus Niger, Fermented cassava gatot, Potato peel waste",

author = "Cindy Angelia and Astia Sanjaya and Aida and Ellen Tanudjaja and Hans Victor and Cahyani, {Antari Daru} and Tan, {Tjie Jan} and Reinhard Pinontoan",

note = "Funding Information: We would like to thank Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UPH for the financial support. Publisher Copyright: {\textcopyright} 2019 Korean Society for Microbiology and Biotechnology. All rights reserved.",

year = "2019",

month = sep,

day = "28",

doi = "10.4014/mbl.1811.11011",

language = "English",

volume = "47",

pages = "364--371",

journal = "Microbiology and Biotechnology Letters",

issn = "1598-642X",

publisher = "Korean Society for Microbiolog and Biotechnology",

number = "3",

}

TY - JOUR

T1 - Characterization of alpha-amylase from aspergillus niger aggregate F isolated from a fermented cassava gatot grown in potato peel waste medium

AU - Angelia, Cindy

AU - Sanjaya, Astia

AU - Aida,

AU - Tanudjaja, Ellen

AU - Victor, Hans

AU - Cahyani, Antari Daru

AU - Tan, Tjie Jan

AU - Pinontoan, Reinhard

N1 - Funding Information: We would like to thank Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UPH for the financial support. Publisher Copyright: © 2019 Korean Society for Microbiology and Biotechnology. All rights reserved.

PY - 2019/9/28

Y1 - 2019/9/28

N2 - The use of GRAS microorganisms isolated from fermented foods during amylase production using an economical food-waste medium provides more opportunities to produce amylase with a wider range of applications. Hence, this study aimed to isolate a good amylase-producing fungi from the traditional Indonesian fermented cassava, gatot, and to identify the amylase-producing capability of the isolate in a potato peel waste (PPW) medium. Black-colored fungi isolated from gatot was morphologically identified and the amylase produced was characterized using SDS-PAGE and Native PAGE. The isolate was then grown on PPW medium, and the amylase produced was further characterized. Morphological identification and enzyme characterization revealed that the Aspergillus niger aggregate F isolated from gatot secreted an active extracellular α-amylase with an optimum pH of 5-6. In conclusion, Aspergillus niger aggregate F isolated from gatot can be used to produce α-amylase using PPW as a medium.

AB - The use of GRAS microorganisms isolated from fermented foods during amylase production using an economical food-waste medium provides more opportunities to produce amylase with a wider range of applications. Hence, this study aimed to isolate a good amylase-producing fungi from the traditional Indonesian fermented cassava, gatot, and to identify the amylase-producing capability of the isolate in a potato peel waste (PPW) medium. Black-colored fungi isolated from gatot was morphologically identified and the amylase produced was characterized using SDS-PAGE and Native PAGE. The isolate was then grown on PPW medium, and the amylase produced was further characterized. Morphological identification and enzyme characterization revealed that the Aspergillus niger aggregate F isolated from gatot secreted an active extracellular α-amylase with an optimum pH of 5-6. In conclusion, Aspergillus niger aggregate F isolated from gatot can be used to produce α-amylase using PPW as a medium.

KW - Alpha-amylase

KW - Aspergillus Niger

KW - Fermented cassava gatot

KW - Potato peel waste

UR - http://www.scopus.com/inward/record.url?scp=85079183453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85079183453&partnerID=8YFLogxK

U2 - 10.4014/mbl.1811.11011

DO - 10.4014/mbl.1811.11011

M3 - Article

AN - SCOPUS:85079183453

SN - 1598-642X

VL - 47

SP - 364

EP - 371

JO - Microbiology and Biotechnology Letters

JF - Microbiology and Biotechnology Letters

IS - 3

ER -

Characterization of alpha-amylase from aspergillus niger aggregate F isolated from a fermented cassava gatot grown in potato peel waste medium

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this