@article{fcf45fee76e54063818e3e0cba452d10,
title = "Characterization of an Extended-Spectrum β-Lactamase from Escherichia coli Mediated by a Plasmid",
abstract = "The β-lactamase from Escherichia coli ML 4953 pKU56 was purified about 190-fold from the crude cell-free extract with an overall recovery of 17%. The molecular weight mediated by pKU56 was about 28,000, and the isoelectric point was about 9.4. This enzyme hydrolyzed cephaloridine, cephalothin, cefamandole, cefuroxime, and cefsulodin at high rate, but ampicillin, piperacillin, and carbenicillin moderately. Antisera against pKU56 enzyme showed partly cross-reaction with β-lactamase from Klebsiella oxytoca and Proteus vulgaris.",
keywords = "extended-spectrum, pKU56, β-lactamase",
author = "Matsuhisa Inoue and Aisaku Fuse and Keizo Yamaguchi and Harushige Kanno and Mitsuo Kaku and Takashi Inamatsu and Tsutomu Nagahama and Masato Yoshida and Masato Nonoyama and Ryoichi Okamoto",
note = "Funding Information: This work was supported by grants 05304028 and 930050 from the Ministry of Education, Science and Culture, JapanWaksman Foundation, and the All Kitasato Project Study (6-2-3g).The following pharmaceutical companies kindly provided the drugs utilized in this study: Cefsulodin (Takeda Pharmaceutical, 'Osaka), cephaloridin, cephalothin, cephalexin, latamoxefand flomoxef( Shionogi Pharmaceutical, Osaka), piperacillin, cefoperazone (Toyama Chemical, Tokyo), cefoxitin (Daiichi Pharmaceutical, Tokyo), cefuroxime (Sinnihon Jit$ugyo,Tokyo), ceftazidime (Tanabe Pharmaceutical,Tokyo), ceftizoxime and carbepicillin (Fujisawa Pharmaceutical, Osaka), aztreonam (Eizai, Tokyo), cefotaxime and cefpirome (Hoechst,Tokyo), cefodizime and tazobactam (Taiho Pharmaceutical, Tokushima), clavulanic acid(Smith Klein Beecham Pharmaceutical,Tokyo), ampicillin (Meiji Seika, Tokyo), sulbactam (pfizer Pharmaceutical, Tokyo), and imipenem,penicillin G and cloxacillin (Banyu Pharmaceutical, Tokyo).",
year = "1995",
doi = "10.1007/BF02347732",
language = "English",
volume = "1",
pages = "70--72",
journal = "Journal of Infection and Chemotherapy",
issn = "1341-321X",
publisher = "Elsevier BV",
number = "1",
}
