Characterization of cellular uptake and distribution of vitamin E

Yoshiro Saito; Yasukazu Yoshida; Keiko Nishio; Mieko Hayakawa; Etsuo Niki

doi:10.1196/annals.1331.047

Characterization of cellular uptake and distribution of vitamin E

Yoshiro Saito, Yasukazu Yoshida, Keiko Nishio, Mieko Hayakawa, Etsuo Niki

PHA - Life and Pharmaceutical Science

National Institute of Advanced Industrial Science and Technology

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

We previously reported that tocotrienols acted as more potent inhibitors against selenium deficiency-induced cell death than the corresponding tocopherol isoforms (J. Biol. Chem. 2003;278:39428-39434). In the present study, we first compared the differences in the cellular uptake between α-tocopherol (α-Toc) and α-tocotrienol (α-Toc-3). The initial rate of cellular uptake of α-Toc-3 was 70-fold higher than that of α-Toc. Subcellular fractionation analysis of α-Toc-3 and α-Toc-fortified cells showed similar cellular distribution of these antioxidants, which was directly proportional to the lipid distribution. The cells containing similar amounts of α-Toc-3 and α-Toc showed similar resistance against the oxidative stress caused by peroxides. These results suggest that the apparent higher cytoprotective effect of α-Toc-3 than α-Toc is primarily ascribed to its higher cellular uptake.

Original language	English
Pages (from-to)	368-375
Number of pages	8
Journal	Annals of the New York Academy of Sciences
Volume	1031
DOIs	https://doi.org/10.1196/annals.1331.047
Publication status	Published - 2004

Keywords

Antioxidant
Cellular uptake
Oxidative stress
Tocotrienol
Vitamin E

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title = "Characterization of cellular uptake and distribution of vitamin E",

abstract = "We previously reported that tocotrienols acted as more potent inhibitors against selenium deficiency-induced cell death than the corresponding tocopherol isoforms (J. Biol. Chem. 2003;278:39428-39434). In the present study, we first compared the differences in the cellular uptake between α-tocopherol (α-Toc) and α-tocotrienol (α-Toc-3). The initial rate of cellular uptake of α-Toc-3 was 70-fold higher than that of α-Toc. Subcellular fractionation analysis of α-Toc-3 and α-Toc-fortified cells showed similar cellular distribution of these antioxidants, which was directly proportional to the lipid distribution. The cells containing similar amounts of α-Toc-3 and α-Toc showed similar resistance against the oxidative stress caused by peroxides. These results suggest that the apparent higher cytoprotective effect of α-Toc-3 than α-Toc is primarily ascribed to its higher cellular uptake.",

keywords = "Antioxidant, Cellular uptake, Oxidative stress, Tocotrienol, Vitamin E",

author = "Yoshiro Saito and Yasukazu Yoshida and Keiko Nishio and Mieko Hayakawa and Etsuo Niki",

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T1 - Characterization of cellular uptake and distribution of vitamin E

AU - Saito, Yoshiro

AU - Yoshida, Yasukazu

AU - Nishio, Keiko

AU - Hayakawa, Mieko

AU - Niki, Etsuo

PY - 2004

Y1 - 2004

N2 - We previously reported that tocotrienols acted as more potent inhibitors against selenium deficiency-induced cell death than the corresponding tocopherol isoforms (J. Biol. Chem. 2003;278:39428-39434). In the present study, we first compared the differences in the cellular uptake between α-tocopherol (α-Toc) and α-tocotrienol (α-Toc-3). The initial rate of cellular uptake of α-Toc-3 was 70-fold higher than that of α-Toc. Subcellular fractionation analysis of α-Toc-3 and α-Toc-fortified cells showed similar cellular distribution of these antioxidants, which was directly proportional to the lipid distribution. The cells containing similar amounts of α-Toc-3 and α-Toc showed similar resistance against the oxidative stress caused by peroxides. These results suggest that the apparent higher cytoprotective effect of α-Toc-3 than α-Toc is primarily ascribed to its higher cellular uptake.

AB - We previously reported that tocotrienols acted as more potent inhibitors against selenium deficiency-induced cell death than the corresponding tocopherol isoforms (J. Biol. Chem. 2003;278:39428-39434). In the present study, we first compared the differences in the cellular uptake between α-tocopherol (α-Toc) and α-tocotrienol (α-Toc-3). The initial rate of cellular uptake of α-Toc-3 was 70-fold higher than that of α-Toc. Subcellular fractionation analysis of α-Toc-3 and α-Toc-fortified cells showed similar cellular distribution of these antioxidants, which was directly proportional to the lipid distribution. The cells containing similar amounts of α-Toc-3 and α-Toc showed similar resistance against the oxidative stress caused by peroxides. These results suggest that the apparent higher cytoprotective effect of α-Toc-3 than α-Toc is primarily ascribed to its higher cellular uptake.

KW - Antioxidant

KW - Cellular uptake

KW - Oxidative stress

KW - Tocotrienol

KW - Vitamin E

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JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

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Characterization of cellular uptake and distribution of vitamin E

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Keywords

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