TY - JOUR
T1 - Characterization of dental epithelial progenitor cells derived from cervical-loop epithelium in a rat lower incisor
AU - Kawano, S.
AU - Saito, M.
AU - Handa, K.
AU - Morotomi, T.
AU - Toyono, T.
AU - Seta, Y.
AU - Nakamura, N.
AU - Uchida, T.
AU - Toyoshima, K.
AU - Ohishi, M.
AU - Harada, H.
PY - 2004/2
Y1 - 2004/2
N2 - Dental epithelial progenitor cells differentiate into various cell types during development of tooth germs. To study this mechanism, we produced immortalized dental epithelial progenitor cells derived from the cervical-loop epithelium of a rat lower incisor. The expression patterns of cytokeratin 14, nerve growth factor receptor p75, amelogenin, Notch2, and alkaline phosphatase were examined by immnohistochemistry in both lower and higher cell densities. The patterns of each were compared in the dental epithelium of rat lower incisors. The results demonstrated that these cells could produce ameloblast lineage cells, stratum intermedium cells, stellate reticulum, and outer enamel epithelium. Furthermore, fibroblast growth factor 10 stimulated proliferation of dental progenitor cells and subsequently increased the number of cells expressing alkaline phosphatase. These results suggest that fibroblast growth factor 10 plays a role in coupling mitogenesis of the cervical-loop cells and the production of stratum intermedium cells in rat incisors.
AB - Dental epithelial progenitor cells differentiate into various cell types during development of tooth germs. To study this mechanism, we produced immortalized dental epithelial progenitor cells derived from the cervical-loop epithelium of a rat lower incisor. The expression patterns of cytokeratin 14, nerve growth factor receptor p75, amelogenin, Notch2, and alkaline phosphatase were examined by immnohistochemistry in both lower and higher cell densities. The patterns of each were compared in the dental epithelium of rat lower incisors. The results demonstrated that these cells could produce ameloblast lineage cells, stratum intermedium cells, stellate reticulum, and outer enamel epithelium. Furthermore, fibroblast growth factor 10 stimulated proliferation of dental progenitor cells and subsequently increased the number of cells expressing alkaline phosphatase. These results suggest that fibroblast growth factor 10 plays a role in coupling mitogenesis of the cervical-loop cells and the production of stratum intermedium cells in rat incisors.
KW - Ameloblasts
KW - Fgf10
KW - Progenitor cells
KW - Rat incisor
KW - Stratum intermedium
UR - http://www.scopus.com/inward/record.url?scp=1942435030&partnerID=8YFLogxK
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U2 - 10.1177/154405910408300209
DO - 10.1177/154405910408300209
M3 - Article
C2 - 14742650
AN - SCOPUS:1942435030
SN - 0022-0345
VL - 83
SP - 129
EP - 133
JO - Journal of Dental Research
JF - Journal of Dental Research
IS - 2
ER -