Characterization of duck microsatellite repeat sequences

Hideaki Takahashi; Masahiro Satoh; Mitsuru Minezawa; Tresnawati Purwadaria; Hardi Prasetyo

doi:10.6090/jarq.35.217

Characterization of duck microsatellite repeat sequences

Hideaki Takahashi, Masahiro Satoh, Mitsuru Minezawa, Tresnawati Purwadaria, Hardi Prasetyo

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We cloned duck microsatellite repeat sequences from genomic DNA of an Indonesian native duck to evaluate the genetic relationships among native breeds of duck. A (CA)_n-enriched library was constructed us ng the method of Takahashi et al. (1996) with modifications. In the library, 8.3% of the clones were CA-positive. The average number of uninterrupted (CA)_n repeats among 22 duck microsatellites was 11.4. Fifteen clones which could be developed as PCR primer pairs to detect (CA)_n, repeat length polymorphisms were identified. They should provide a valuable tool for studies related to biodiversity and population structure in duck.

Original language	English
Pages (from-to)	217-219
Number of pages	3
Journal	Japan Agricultural Research Quarterly
Volume	35
Issue number	4
DOIs	https://doi.org/10.6090/jarq.35.217
Publication status	Published - 2001 Oct

Keywords

DNA
Indonesia

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10.6090/jarq.35.217

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abstract = "We cloned duck microsatellite repeat sequences from genomic DNA of an Indonesian native duck to evaluate the genetic relationships among native breeds of duck. A (CA)n-enriched library was constructed us ng the method of Takahashi et al. (1996) with modifications. In the library, 8.3% of the clones were CA-positive. The average number of uninterrupted (CA)n repeats among 22 duck microsatellites was 11.4. Fifteen clones which could be developed as PCR primer pairs to detect (CA)n, repeat length polymorphisms were identified. They should provide a valuable tool for studies related to biodiversity and population structure in duck.",

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author = "Hideaki Takahashi and Masahiro Satoh and Mitsuru Minezawa and Tresnawati Purwadaria and Hardi Prasetyo",

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AU - Takahashi, Hideaki

AU - Satoh, Masahiro

AU - Minezawa, Mitsuru

AU - Purwadaria, Tresnawati

AU - Prasetyo, Hardi

PY - 2001/10

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N2 - We cloned duck microsatellite repeat sequences from genomic DNA of an Indonesian native duck to evaluate the genetic relationships among native breeds of duck. A (CA)n-enriched library was constructed us ng the method of Takahashi et al. (1996) with modifications. In the library, 8.3% of the clones were CA-positive. The average number of uninterrupted (CA)n repeats among 22 duck microsatellites was 11.4. Fifteen clones which could be developed as PCR primer pairs to detect (CA)n, repeat length polymorphisms were identified. They should provide a valuable tool for studies related to biodiversity and population structure in duck.

AB - We cloned duck microsatellite repeat sequences from genomic DNA of an Indonesian native duck to evaluate the genetic relationships among native breeds of duck. A (CA)n-enriched library was constructed us ng the method of Takahashi et al. (1996) with modifications. In the library, 8.3% of the clones were CA-positive. The average number of uninterrupted (CA)n repeats among 22 duck microsatellites was 11.4. Fifteen clones which could be developed as PCR primer pairs to detect (CA)n, repeat length polymorphisms were identified. They should provide a valuable tool for studies related to biodiversity and population structure in duck.

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JO - Japan Agricultural Research Quarterly

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Characterization of duck microsatellite repeat sequences

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Keywords

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