Characterization of local respiratory activity of PC12 neuronal cell by scanning electrochemical microscopy

Yuki Takii; Kimiyasu Takoh; Matsuhiko Nishizawa; Tomokazu Matsue

doi:10.1016/S0013-4686(03)00408-0

Characterization of local respiratory activity of PC12 neuronal cell by scanning electrochemical microscopy

Yuki Takii, Kimiyasu Takoh, Matsuhiko Nishizawa, Tomokazu Matsue

Tohoku University

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

Scanning electrochemical microscopy (SECM) was applied to the study of the spatial distribution of the respiratory activity in a PC12 neuronal cell. The SECM imaging of the oxygen concentration around the cells indicated that the axon of PC12 as well as its cell body consumed oxygen as the result of mitochondrial respiration. The variation in respiratory activity during NGF-induced axonal growth was monitored at the growth cone of the axon. The respiratory activity at the growth cone was found to be particularly activated by the NGF treatment.

Original language	English
Pages (from-to)	3381-3385
Number of pages	5
Journal	Electrochimica Acta
Volume	48
Issue number	20-22
DOIs	https://doi.org/10.1016/S0013-4686(03)00408-0
Publication status	Published - 2003 Sept 30

Keywords

Mitochondrial distribution
NGF
PC12
Respiratory activity
Scanning electrochemical microscopy

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10.1016/S0013-4686(03)00408-0

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title = "Characterization of local respiratory activity of PC12 neuronal cell by scanning electrochemical microscopy",

abstract = "Scanning electrochemical microscopy (SECM) was applied to the study of the spatial distribution of the respiratory activity in a PC12 neuronal cell. The SECM imaging of the oxygen concentration around the cells indicated that the axon of PC12 as well as its cell body consumed oxygen as the result of mitochondrial respiration. The variation in respiratory activity during NGF-induced axonal growth was monitored at the growth cone of the axon. The respiratory activity at the growth cone was found to be particularly activated by the NGF treatment.",

keywords = "Mitochondrial distribution, NGF, PC12, Respiratory activity, Scanning electrochemical microscopy",

author = "Yuki Takii and Kimiyasu Takoh and Matsuhiko Nishizawa and Tomokazu Matsue",

note = "Funding Information: The present work was supported by grants-in-aid for Scientific Research on Priority Area (no. 1122720) and B (no. 13450348) from the Ministry of Education, Science and Culture, Japan.",

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doi = "10.1016/S0013-4686(03)00408-0",

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T1 - Characterization of local respiratory activity of PC12 neuronal cell by scanning electrochemical microscopy

AU - Takii, Yuki

AU - Takoh, Kimiyasu

AU - Nishizawa, Matsuhiko

AU - Matsue, Tomokazu

N1 - Funding Information: The present work was supported by grants-in-aid for Scientific Research on Priority Area (no. 1122720) and B (no. 13450348) from the Ministry of Education, Science and Culture, Japan.

PY - 2003/9/30

Y1 - 2003/9/30

N2 - Scanning electrochemical microscopy (SECM) was applied to the study of the spatial distribution of the respiratory activity in a PC12 neuronal cell. The SECM imaging of the oxygen concentration around the cells indicated that the axon of PC12 as well as its cell body consumed oxygen as the result of mitochondrial respiration. The variation in respiratory activity during NGF-induced axonal growth was monitored at the growth cone of the axon. The respiratory activity at the growth cone was found to be particularly activated by the NGF treatment.

AB - Scanning electrochemical microscopy (SECM) was applied to the study of the spatial distribution of the respiratory activity in a PC12 neuronal cell. The SECM imaging of the oxygen concentration around the cells indicated that the axon of PC12 as well as its cell body consumed oxygen as the result of mitochondrial respiration. The variation in respiratory activity during NGF-induced axonal growth was monitored at the growth cone of the axon. The respiratory activity at the growth cone was found to be particularly activated by the NGF treatment.

KW - Mitochondrial distribution

KW - NGF

KW - PC12

KW - Respiratory activity

KW - Scanning electrochemical microscopy

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JO - Electrochimica Acta

JF - Electrochimica Acta

IS - 20-22

ER -

Characterization of local respiratory activity of PC12 neuronal cell by scanning electrochemical microscopy

Abstract

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