TY - JOUR
T1 - Characterization of methanogenesis, acidogenesis and hydrolysis in thermophilic methane fermentation of chicken manure
AU - Niu, Qigui
AU - Hojo, Toshimasa
AU - Qiao, Wei
AU - Qiang, Hong
AU - Li, Yu You
N1 - Funding Information:
The authors would like to acknowledge the technical cooperation in this investigation from Hitachi Engineering and Serves Co. Ltd. The first author was supported by China Scholarship Council (CSC) for a scholarship.
PY - 2014/5/15
Y1 - 2014/5/15
N2 - The thermophilic methane fermentation of chicken manure (CM) with a total solids (TS) of approximately 10% was investigated with regard to ammonia inhibition. A gradual increase in total ammonia nitrogen to 6000mg/L was observed in the case of raw CM fermentation. A distinct rise in VFA accumulation combined with a low methane production of 0.29L/gVS occurred at a TAN of 4000~5000mg/L. Biogas production completely ceased when TAN reached 8000mg/L after the addition of NH4HCO3. The high sensitivity of methanogenesis to TAN and free ammonia (FA) was quantitatively confirmed. The IC50 of TAN for methanogenesis, acidogenesis and hydrolysis was 5058, 5305 and 5707mg/L at a pH value of 8.1±0.2. Similar results but with a lower IC50 were obtained for FA inhibition during fermentation. The microbial community analysis revealed significant differences in hydrogenotrophic methanogens and aceticlastic methanogens before and after inhibition.
AB - The thermophilic methane fermentation of chicken manure (CM) with a total solids (TS) of approximately 10% was investigated with regard to ammonia inhibition. A gradual increase in total ammonia nitrogen to 6000mg/L was observed in the case of raw CM fermentation. A distinct rise in VFA accumulation combined with a low methane production of 0.29L/gVS occurred at a TAN of 4000~5000mg/L. Biogas production completely ceased when TAN reached 8000mg/L after the addition of NH4HCO3. The high sensitivity of methanogenesis to TAN and free ammonia (FA) was quantitatively confirmed. The IC50 of TAN for methanogenesis, acidogenesis and hydrolysis was 5058, 5305 and 5707mg/L at a pH value of 8.1±0.2. Similar results but with a lower IC50 were obtained for FA inhibition during fermentation. The microbial community analysis revealed significant differences in hydrogenotrophic methanogens and aceticlastic methanogens before and after inhibition.
KW - Chicken manure
KW - Methanogenesis
KW - Microbial community
KW - Thermophilic methane fermentation
KW - Wide range of ammonia
UR - http://www.scopus.com/inward/record.url?scp=84896854406&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896854406&partnerID=8YFLogxK
U2 - 10.1016/j.cej.2013.11.074
DO - 10.1016/j.cej.2013.11.074
M3 - Article
AN - SCOPUS:84896854406
SN - 1385-8947
VL - 244
SP - 587
EP - 596
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
ER -