Characterization of microbial community in the two-stage process for hydrogen and methane production from food waste

Chun Feng Chu, Yoshitaka Ebie, Kai Qin Xu, Yu You Li, Yuhei Inamori

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)


The structure of a microbial community in the two-stage process for H 2 and CH4 production from food waste was investigated by a molecular biological approach. The process was a continuous combined thermophilic acidogenic hydrogenesis and mesophilic (RUN1) or thermophilic (RUN2) methanogenesis with recirculation of the digested sludge. A two-phase process suggested in this study effectively separate H2-producing bacteria from methanogenic archaea by optimization of design parameters such as pH, hydraulic retention time (HRT) and temperature. Galore microbial diversity was found in the thermophilic acidogenic hydrogenesis, Clostridium sp. strain Z6 and Thermoanaerobacterium thermosaccharolyticum were considered to be the dominant thermophilic H2-producing bacteria. The hydrogenotrophic methanogens were inhibited in thermophilic methanogenesis, whereas archaeal rDNAs were higher in the thermophilic methanogenesis than those in mesophilic methanogenesis. The yields of H2 and CH4 were in equal range depending on the characteristics of food waste, whereas effluent water quality indicators were different obviously in RUN1 and RUN2. The results indicated that hydrolysis and removal of food waste were higher in RUN2 than RUN1.

Original languageEnglish
Pages (from-to)8253-8261
Number of pages9
JournalInternational Journal of Hydrogen Energy
Issue number15
Publication statusPublished - 2010 Aug


  • Bio-hydrogen
  • Bio-methane
  • Cloning
  • Real-time PCR
  • Two-stage process


Dive into the research topics of 'Characterization of microbial community in the two-stage process for hydrogen and methane production from food waste'. Together they form a unique fingerprint.

Cite this