TY - JOUR
T1 - Characterization of middle-molecule introduction into cells using mm-scale discharge in saline
AU - Honda, Ryosuke
AU - Sasaki, Shota
AU - Takashima, Keisuke
AU - Kanzaki, Makoto
AU - Sato, Takehiko
AU - Kaneko, Toshiro
N1 - Publisher Copyright:
© 2020 The Japan Society of Applied Physics.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - While gas-phase atmospheric pressure plasma (APP) is a promising technology for highly-efficient and minimally-invasive gene/drug (molecule) introduction, the reduction of external gas supply and the expansion of the effective treatment area in biological fluids for in vivo treatment are still challenging. We developed a device for mm-scale plasma generation in saline without working gas supply and demonstrated the local introduction of YOYO-1 molecule (molecular weight : 1271) into cultured cells using the device. Unlike the conventional APP accounting for chemical stimuli with reactive species, plasma-induced mechanical stimulus was indicated as one of key factor(s) in the molecule introduction.
AB - While gas-phase atmospheric pressure plasma (APP) is a promising technology for highly-efficient and minimally-invasive gene/drug (molecule) introduction, the reduction of external gas supply and the expansion of the effective treatment area in biological fluids for in vivo treatment are still challenging. We developed a device for mm-scale plasma generation in saline without working gas supply and demonstrated the local introduction of YOYO-1 molecule (molecular weight : 1271) into cultured cells using the device. Unlike the conventional APP accounting for chemical stimuli with reactive species, plasma-induced mechanical stimulus was indicated as one of key factor(s) in the molecule introduction.
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U2 - 10.35848/1347-4065/ab7d7e
DO - 10.35848/1347-4065/ab7d7e
M3 - Article
AN - SCOPUS:85083321894
SN - 0021-4922
VL - 59
JO - Japanese Journal of Applied Physics
JF - Japanese Journal of Applied Physics
IS - 4
M1 - 040904
ER -