Characterization of newly established bovine intestinal epithelial cell line

Kohtaro Miyazawa, Tetsuya Hondo, Takashi Kanaya, Sachi Tanaka, Ikuro Takakura, Wataru Itani, Michael T. Rose, Haruki Kitazawa, Takahiro Yamaguchi, Hisashi Aso

    Research output: Contribution to journalArticlepeer-review

    52 Citations (Scopus)


    Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells.

    Original languageEnglish
    Pages (from-to)125-134
    Number of pages10
    JournalHistochemistry and Cell Biology
    Issue number1
    Publication statusPublished - 2010 Jan


    • Bovine small intestine
    • Cell differentiation
    • Epithelial cell line
    • M cells
    • Peyer's patches

    ASJC Scopus subject areas

    • Histology
    • Molecular Biology
    • Medical Laboratory Technology
    • Cell Biology


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