Characterization of nika histidine kinase and two response regulators with special reference to osmotic adaptation and asexual development in aspergillus nidulans

Daisuke Hagiwara, Takeshi Mizuno, Keietsu Abe

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25 Citations (Scopus)


Aspergillus nidulans has many histidine-to-aspartate (His-Asp) phosphorelay components, including 15 histidine kinases (HKs), four response regulators (RRs), and a histidine-containing phosphotransfer intermediate (HPt). Of these, NikA (HK) is highly conserved in many filamentous fungi. It has been found that NikA is responsible for the responses of filamentous fungi to fungicides such as iprodione and fludioxonil. Two RRs, SskA and SrrA, are also involved in the fungicide response, providing a typical example of the His-Asp phosphorelay system, in which NikA functions as a sensor upstream of SskA and SrrA in response to fungicides. To gain further insight into the physiological roles of the NikA-SskA/SrrA phosphorelay system, we constructed a pair of δnikAδsskA and δnikAδsrrA double mutants. Here we provide evidence regarding the crucial involvement of the NikA-SskA/SrrA phosphorelay system in both osmotic adaptation and asexual development, including conidia formation. Based on these results, a general insight into the A. nidulans His- Asp phosphorelay network is also discussed.

Original languageEnglish
Pages (from-to)1566-1571
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Issue number7
Publication statusPublished - 2009


  • Asexual development
  • Aspergillus nidulans
  • Histidine-to-aspartate (his-asp) phosphorelay
  • NikA histidine kinase
  • Osmotic stress response

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