TY - JOUR
T1 - Characterization of peptides in sea anemone venom collected by a novel procedure
AU - Malpezzi, Elena L.A.
AU - de Freitas, JoséCarlos
AU - Muramoto, Koji
AU - Kamiya, Hisao
N1 - Funding Information:
Acknowledgements-This project was supported by FundarAo de Amparo i Pesquisa do Estado de Silo Paulo, Brazil (FAPESP, Prroc. no.89/26949 and 91/3349-3). Theauthors gratefully acknowledge Dr LASZLO BÉRFSS of InstituteofToxicology, University of Kiel, Germany, for the gift of ATX-II, and Drs E. G. MENDEs and R. J. HuxTABLE for carefully reading the manuscript.
PY - 1993/7
Y1 - 1993/7
N2 - Peptide neurotoxins were isolated from the venom obtained by electrical stimulation of the sea anemone Bunodosoma caissarum. This technique allows almost pure venom to be collected, and the animals to survive. Three neurotoxins (assayed on crustacean nerves) were isolated by gel filtration and reversed-phase high performance liquid chromatography. Hemolysins were also detected in the venom. The amino acid sequence of a major neurotoxin BcIII was determined. BcIII has 48 amino acid residues with six half-cystine residues. This sequence has homology with the type 1 long sea anemone neurotoxins. Two minor toxins (BcI and II) have similar amino acid composition and amino-terminal sequences to BcIII.
AB - Peptide neurotoxins were isolated from the venom obtained by electrical stimulation of the sea anemone Bunodosoma caissarum. This technique allows almost pure venom to be collected, and the animals to survive. Three neurotoxins (assayed on crustacean nerves) were isolated by gel filtration and reversed-phase high performance liquid chromatography. Hemolysins were also detected in the venom. The amino acid sequence of a major neurotoxin BcIII was determined. BcIII has 48 amino acid residues with six half-cystine residues. This sequence has homology with the type 1 long sea anemone neurotoxins. Two minor toxins (BcI and II) have similar amino acid composition and amino-terminal sequences to BcIII.
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U2 - 10.1016/0041-0101(93)90220-D
DO - 10.1016/0041-0101(93)90220-D
M3 - Article
C2 - 8105563
AN - SCOPUS:0027316842
SN - 0041-0101
VL - 31
SP - 853
EP - 864
JO - Toxicon
JF - Toxicon
IS - 7
ER -