TY - CHAP
T1 - Characterization of STRO-1 positive cells derived from human wisdom tooth germs
AU - Iwamatsu-Kobayashi, Yoko
AU - Nishihara, Daisuke
AU - Endo, Naoki
AU - Kindaichi, Koji
AU - Kindaichi, Junko
AU - Komatsu, Masashi
N1 - Publisher Copyright:
© Springer 2012.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Recently, the research on the stem cells is developing rapidly in the odontology. Mesenchymal stem cells (MSCs) have the possibility useful for the stem cell treatment and the tissue engineering. STRO-1 is one of the mesenchymal stem cell markers. In this study, we examined the characterization of STRO-1 posi- tive cells derived from human wisdom tooth germs (hWTGs) compared with STRO-1 negative cells. As positive control, we used human mesenchymal stem cells (HMSC) on purchase. STRO-1 positive cells derived from hWTGs showed Alcian blue positive matrix like HMSC when cultured in chondrogenic induction medium. STRO-1 negative cells from hWTGs did not show such matrix. Nodule formation stained with Alizarin red was performed not only by STRO-1 positive cells but also STRO-1 negative cells cultured in osteogenic induction medium. It is suggested that STRO-1 positive cells derived from hWTGs are useful for the stem cell treatment and the tissue engineering.
AB - Recently, the research on the stem cells is developing rapidly in the odontology. Mesenchymal stem cells (MSCs) have the possibility useful for the stem cell treatment and the tissue engineering. STRO-1 is one of the mesenchymal stem cell markers. In this study, we examined the characterization of STRO-1 posi- tive cells derived from human wisdom tooth germs (hWTGs) compared with STRO-1 negative cells. As positive control, we used human mesenchymal stem cells (HMSC) on purchase. STRO-1 positive cells derived from hWTGs showed Alcian blue positive matrix like HMSC when cultured in chondrogenic induction medium. STRO-1 negative cells from hWTGs did not show such matrix. Nodule formation stained with Alizarin red was performed not only by STRO-1 positive cells but also STRO-1 negative cells cultured in osteogenic induction medium. It is suggested that STRO-1 positive cells derived from hWTGs are useful for the stem cell treatment and the tissue engineering.
KW - Chondrogenic induction
KW - Human wisdom tooth germ
KW - Mesenchymal stem cell
KW - Osteogenic induction
KW - STRO-1
UR - http://www.scopus.com/inward/record.url?scp=84957011240&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84957011240&partnerID=8YFLogxK
U2 - 10.1007/978-4-431-54070-0_27
DO - 10.1007/978-4-431-54070-0_27
M3 - Chapter
AN - SCOPUS:84957011240
SN - 9784431540694
SP - 119
EP - 121
BT - Interface Oral Health Science 2011
PB - Springer Japan
ER -